摘要
目的观察氨氯地平对氧化型低密度脂蛋白(ox-LDL)诱导的人脐静脉内皮细胞(HUVEC-12)中白细胞分化抗原CD40表达的影响,从一个新的角度探讨氨氯地平的抗动脉粥样硬化作用机制。方法实验分为6组:对照组、10.0μmol/L氨氯地平单独处理组、ox-LDL组、0.1、1.0及10.0μmol/L氨氯地平预先处理细胞1 h后加入50 mg/L ox-LDL共同孵育24 h组。用逆转录聚合酶链反应(RT-PCR)检测CD40 mRNA的表达,W estern b lot检测CD40蛋白的表达。结果随着氨氯地平浓度增加,细胞CD40的mRNA和蛋白表达逐渐降低,当浓度为10.0μmol/L时作用更明显。结论氨氯地平对ox-LDL诱导的HUVEC-12细胞CD40的表达具有抑制作用。
Aim To study the effect of Amlodipine on CD40 expression in HUVEC-12 endothelial cells induced by oxidized low density lipoprotein(ox-LDL) to explore the new antiatherosclerotic role of Amlodipine. Methods The experiment groups included 6 groups: control group,10.0 μmol/L Amlodipine alone treated group,ox-LDL group,and different concentration(0.1,1.0,10.0 μmol/L) of Amlodipine pretreated for 1 h and treated with 50 mg/L ox-LDL for 24 h group.RT-PCR was used to detect the mRNA expression of CD40 and western-blot was used to detect the protein expression of CD40. Results RT-PCR and western blot showed that with ox-LDL and Amlodipine treatment,both mRNA and protein expression of CD40 were greatly decreased in a concentration-dependent manner. Conclusion Amlodipine can have a significant effect on down-regulating CD40 expression induced by ox-LDL.
出处
《中国动脉硬化杂志》
CAS
CSCD
北大核心
2011年第10期809-812,共4页
Chinese Journal of Arteriosclerosis
基金
湖南省卫生厅科技项目(B2007092
2007101)
衡阳市科学技术发展项目(2010kj17)