摘要
本文建立了葛花中6″-O-木糖鸢尾苷含量测定的HPLC方法。采用GRACE C18(250 mm×4.6 mm,5μm)色谱柱,流动相为乙腈-水梯度洗脱,流速为0.8 mL/min,紫外检测波长为265 nm,柱温为室温。6″-O-木糖鸢尾苷的峰面积(Y)与浓度(X)在10.33～185.99μg/mL范围内线性关系良好,Y=30731X–216635,r=0.9992(n=7);平均回收率为100.2%,RSD<2%(n=9);测得8批葛花药材中6″-O-木糖鸢尾苷含量为11.08～48.23 mg/g。方法学验证结果表明该法准确、可靠,可用于葛花中主要成分6″-O-木糖鸢尾苷的含量测定。
An HPLC method for determination of 6＂-O-xylosyl-tectoridin in Flos Puerariae was developed. The samples were separated on a Cls （250 mm x 4.6 ram,5 μm） column with the mobile phase of aeetonitrile-water （ gradient elution ）. Flow rate was 0.8 mL/min. The detection wavelength was set at 265 nm. Column temperature was ambient temperature. The linear range of 6＂-O-xylosyl-tectoridin was 10.33-185.99 μg/mL, and the linear function was Y = 30731X - 216635 （r = 0.9992）. The average recovery was 100.2% , and RSD was less than 2% （ n = 9）. The contents of 6＂-0- xylosyl-tectoridin in Flos Puerariae was 11.08-48.23 mg/g. The method was accurate and reliable, which can be used to evaluate the qualities of Flos Puerariae.
出处
《天然产物研究与开发》
CAS
CSCD
2011年第5期898-900,938,共4页
Natural Product Research and Development
基金
中山大学2008年实验室开放基金项目″葛花异黄酮类成分的药动/药效学研究″资助
关键词
葛花
6"-O-木糖鸢尾苷
高效液相色谱法
解酒
不同产地
Flos Puerariae
6＂-O-xylosyl-tectoridin
HPLC
hangover alleviating
different origins of production
