摘要
利用正相硅胶、葡聚糖凝胶Sephadex LH-20和反相C18柱层析等方法,从海绵共附生疣孢菌FIM06031的发酵菌丝体提取液中分离到三个化合物(1～3)。通过波谱方法鉴定其中一个化合物harrucomicin C(1)为新倍半萜,另外两个已知化合物为cyperusol C(2)和Nb-乙酰色胺(3)。活性研究表明harrucomicin C对肿瘤细胞株HepG2、EC109和HeLa具显著增殖抑制活性,其IC50值分别为16.99、25.33μM和34.64μM;cyperusol C对肿瘤细胞HeLa和HepG2增殖抑制作用的IC50值分别为149.99μM和167.78μM。
Three compounds （1-3） were isolated from culture broth of Verrucosispora sp. FIM06031 from marine sponge by silica gel, sephadex LH-20 and Cls reversed phase column chromatography. On the basis of spectral data,they were identified as a novel sesquiterpenoid harrucomicin C （1）, eyperusol C （2）, and Nb-acetyhryptamine （3）. Compound 1 showed prominent growth inhibitory activity against the tumor cell fines HepG2, EC109 and HeLa with IC50 values of 16. 99,25.33 μM and 34.64 p,M, respectivity in vitro by MTT assay, while 2 showed growth inhibitory activity against HeLa and HepG2 ceils with IC50 values of 149.99 μM and 167.78 μM,respectively.
出处
《天然产物研究与开发》
CAS
CSCD
2011年第5期797-800,共4页
Natural Product Research and Development
基金
国家“十一五”重大专项(2010ZX09401-403)
福建省自然科学基金项目(2011J01092)
福建省重点项目(2009R10003-1)
