摘要
以M-NH2-SiO2为载体,戊二醛为交联剂制备固定化漆酶,研究2,4-DCP(2,4-二氯苯酚)浓度、溶液酸度、温度对固定化漆酶降解2,4-DCP的影响.结果表明,固定化漆酶降解2,4-DCP最佳条件为:当2,4-DCP质量浓度为5mg.L-1,溶液pH为5.5,温度为30℃时,降解去除率为42.28%.固定化漆酶具有最大活性时的溶液pH值相比于自由酶的最佳pH向碱性偏移;与自由漆酶相比,固定化漆酶有良好的稳定性和重复使用性.
Laccase was immobilized on M-NH2-SiO2 with glutaraldehyde as crosslinking agent.The effects on the activity of immobilized laccase,such as concentration of 2,4-DCP（2,4-dichlorophenols）,acidity of solution,temperature were studied.The results show when concentration of 2,4-DCP is 5 mg·L-1,pH of solution is 5.5,and the temperature is at 30℃,the highest removal rate of 2,4-DCP is 42.28%.The results show that the optimal pH for immobilized laccase is higher than the best pH for free enzyme.Compared with the free enzyme,its stability and repetition are considerably improved.
出处
《同济大学学报（自然科学版）》
EI
CAS
CSCD
北大核心
2011年第9期1324-1328,1393,共6页
Journal of Tongji University:Natural Science
基金
国家自然科学基金(20971043
20577010)
