摘要
目的检测mcpr1基因的蛋白在正常腭突及小鼠腭裂模型腭突发育中的时空表达模式。方法实验组管饲含全反式维甲酸的植物油诱导建立小鼠腭裂模型,对照组小鼠管饲植物油。2组取胎龄12、13、14、16 d的胎鼠各3只,包埋切片,HE染色观察2组胎鼠腭部发育的形态学变化,免疫组织化学方法观察MCPR1蛋白在2组胎鼠腭突中的表达差异。结果成功建立小鼠腭裂模型。切片HE染色观察发现实验组双侧腭突体积较对照组小,两侧腭突未接触;而对照组腭突融合,腭骨形成。免疫组织化学检查结果表明对照组小鼠胎龄12 d时MCPR1蛋白在腭突上皮细胞呈强阳性表达;而实验组小鼠胎龄12 d时腭突间充质细胞内MCPR1蛋白的表达较强,实验组胎龄16 d小鼠仅在上皮细胞附近的间充质细胞中有阳性表达。同一时间点比较,MCPR1蛋白的表达在实验组均强于对照组(P<0.05)。结论 MCPR1蛋白的表达变化随腭部发育存在时空表达差异。
Objective To determine the expression pattern of mcpr1 in murine palatogenesis between the normal murine palatal shelves and the cleft palatal ones.Methods The murine embryonic model with cleft palate was established by inducing with retinoic acid.Immunohistochemical staining was used to detect the expression of mcpr1 gene during the murine embryonic development.Results The embryonic mouse with cleft palate was established successfully.At embryonic day 12（E12）,when palatal shelves have elongated and grown vertically,MCPR1 was expressed strongly in epithelial cells in normal palatal shelves,and in mesenchymal cells in cleft palatal shelves.At E16,MCPR1 was expressed in the mesenchymal cells near the epithelial components.At the corresponding time points,the expression of MCPR1 protein was stronger in normal palatal shelves than in cleft ones respectively.Conclusion The expression of MCPR1 protein was different between the normal and the cleft palate during the palatalogenesis.
出处
《广东牙病防治》
2011年第9期455-458,共4页
Journal of Dental Prevention and Treatment
基金
国家自然科学基金青年科学基金项目(30901691)
关键词
腭裂
腭突
mcpr1基因
免疫组化
Cleft palate
Palatal shelves
mcpr1 gene
Immunohistochemistry
