摘要
目的:采用HPLC测定营养强化剂类保健食品中生物素的含量。方法:样品采用水溶剂超声波提取方法,以Symmetry Shield RPC18柱为固定相,乙腈:0.05%三氟乙酸水溶液(pH=2.5)为流动相,检测波长为201 nm。结果:生物素与其它杂质均能达到基线分离,其色谱峰形好。生物素的浓度在2.0μg/ml～40.0μg/ml时与峰面积成良好的线性关系(Y=-666+1.02×105X,r=0.9999),平均回收率为94.5%～105%,方法精密度RSD为1.93%、和1.47%(n=6)。结论:所用方法简便,准确,重复性好,适用于营养强化剂类保健食品中生物素的测定。
Objective： To determine the level of biotin in health-care food of nutrition enhancers by high performance liquid chromatography.Methods： The samples were extracted by ultrasonic wave.The stationary phase was a Symmetry Shield RPC18 column and the mobile phase was 0.05% trifluoroacetic acid solution（pH 2.5）.The detective wavelength was set at 201 nm.Results： Baseline separation was achieved for biotin and well-shaped chromatograph peak was obtained in the experiment.The calibration curve were linear over the range of 2.0 μg/ml～40.0 μg/ml（Y=-666＋1.02×105X,r=0.9999）.The average recoveries ranged from 94.5% to 105% and the relative standard deviations（RSD） were evaluated to be 1.93% and 1.47%（n=6）.Conclusion： The proposed method has the advantages of simple,convenient,accurate and good repeatability.It was suitable for the determination of biotin in health-care food of nutrition enhancers.
出处
《中国卫生检验杂志》
CAS
2011年第9期2160-2161,共2页
Chinese Journal of Health Laboratory Technology
关键词
生物素
高效液相色谱法
营养强化剂
保健食品
Biotin
High performance liquid chromatography
Nutrition enhancers
Health-care food
