摘要
目的观察慢性阻塞性肺疾病(COPD)大鼠心、肝、脾、肺、肠等多脏器病理改变及"宣肺"中药对其的影响,为探讨"肺合大肠"中医脏腑相关理论奠定基础。方法熏烟加气管滴注脂多糖法复制COPD大鼠模型,观察其心、肝、脾、肺、肾、肠、睾丸、膀胱等组织病理学改变,检测支气管肺泡灌洗液中肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)含量,观察小肠推进率、肠组织IFN-γI、L-10 mRNA基因表达及宣肺中药的干预效果。结果 COPD模型组大鼠支气管上皮损伤及部分脱落,肺泡紊乱,肺泡间质增厚,管内大量炎性细胞浸润,部分肺泡实变;支气管肺泡灌洗液中TNF-αI、L-1β含量、淋巴细胞计数明显升高;结肠组织黏膜层腺体排列欠整齐紧凑、淋巴细胞增多;同时伴见小肠推进率降低,结肠组织IFN-γmRNA基因表达升高I、L-10 mRNA基因表达降低等改变。心、肝、脾等其他脏器组织无明显病理改变。宣肺中药干预后肺组织病理学呈现改善征象,支气管肺泡灌洗液中淋巴细胞计数、炎症因子TNF-αI、L-1β含量均明显降低;同时,直肠组织病理改变减轻,小肠推进率改善,肠组织匀浆中IFN-γmRNA基因表达降低I、L-10 mRNA基因表达升高。结论熏烟加气管滴注脂多糖复制COPD模型存在着特异性的肠道病理改变,而应用宣肺中药从肺论治COPD,肺组织病理改变减轻的同时伴见肠组织病理改变呈改善趋势,从侧面印证了中医"肺合大肠"脏腑相关理论,肺-肠之间存在着一定的相关性及互动调节现象。
Objective To observe with chronic obstructive pulmonary the pathological changes of heart, disease （COPD）, and influence liver, spleen and intestines in rats of Chinese medicinal with action of diffusing lung, and to lay a foundation for the study on TCM theory of lung associating with large intestine. Methods The rat model of COPD was established by using intratracheal drip transfusion of LPS and cigarette smoking. The pathological changes of heart, liver, spleen, lung, kidney, intestines, testis and bladder were observed. The content of tumor necrosis factor-α （TNF-α） and interleukin-1 （IL-1β） in bronchoalveolar lavage fluid （BALF）, gastric emptying, expressions of IFN-γ mRNA and IL- 10 mRNA in intestine tissue and intervention effect of Chinese medicinal were detected and observed. Results In the model group, lesion and partial exuviation of bronchiolar epithetium, adveolar disorder and interstitial thickening, infiltration of a large number of inflammatory cells and consolidation of partial alveoli were observed. The content of TNF-α and IL-1β and lymphocyte count increased significantly. The arrangement of colonic mucous glands was irregular and non-compact, lymphocytes increased, gastric emptying decreased, the expression of colonic IFN-γmRNA increased, and expression of IL-IO mRNA decreased. There were no obvious pathological changes observed in heart, liver and spleen. After the intervention of Chinese medicinal with the action of diffusing lung, the pathological changes were alleviated in lung, lymphocyte count and the content of TNF-α and IL-1 β in BALF decreased significantly. At the same time, the pathological changes were alleviated in colon, gastric emptying was improved, expression of IFN-γ mRNA decreased and expression of IL-10 mRNA increased in colonic homogenate. Conclusion The model of COPD has specific pathological changes in intestines. The administration of Chinese medicinal with the action of diffusing lung can alleviat6 the pathological changes in lung and intest
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2011年第9期591-594,I0001,共5页
Journal of Beijing University of Traditional Chinese Medicine
基金
国家自然科学基金项目(No.30873209)
关键词
慢性阻塞性肺疾病
脏腑相关
肺合大肠
大鼠
chronic obstructive pulmonary disease
correlation between zang-organs and fu-organs
lungassociating with large intestine
rats
