摘要
将氧化苦参碱、甘草酸、黄芪多糖、栀子苷、绿原酸、虎杖苷和木犀草素等7种中药成分稀释成安全浓度范围内的高、中、低3种浓度,分别与NDV以3种方式加入到培养成单层的CEF的培养体系中,用MTT法测定NDV感染细胞能力的变化。结果表明,先加中药后加病毒时,氧化苦参碱、甘草酸、黄芪多糖的高、中浓度、栀子苷高浓度显著抑制病毒感染细胞;先加病毒后加中药时,氧化苦参碱的高、中浓度、黄芪多糖的中、低浓度显著抑制病毒感染细胞;中药与病毒同时加入时,氧化苦参碱的高、中浓度,黄芪多糖的高、中、低浓度和甘草酸高浓度显著抑制病毒感染细胞。绿原酸、虎杖苷和木犀草素3种中药成分无论是哪种加药方式均无抑制NDV感染细胞的作用。
Seven CHMIs were diluted into high,middle and low concentrations within safety concentrations and added into CEF with Newcastle disease virus（NDV） in three different ways（pre-,post-and synchronously）.The effects of seven CHMIs on the infectivity of NDV were assayed by MTT method.The results show that OM,GA and APS at high and middle doses and GP at high dose could inhibit NDV from infecting CEF significantly if pre-added.OM at high and middle doses and APS at middle and low doses could inhibit infectivity obviously if post-added.APS at high,middle and low doses,OM at high and middle doses,and GA at high dose all could inhibit infectivity significantly in simultaneous adding CHMIs and NDV.While CA,PD and LT had no effect.
出处
《中国兽医杂志》
CAS
北大核心
2011年第9期46-48,49,共4页
Chinese Journal of Veterinary Medicine
基金
国家科技支撑计划(2008BADB4B06)
江苏青蓝工程骨干教师资助项目
江苏楠森动物保健有限公司与江苏畜牧兽医职业技术学院横向联合课题(200836)
关键词
中药成分
病毒感染细胞能力
抗病毒作用
Chinese herbal medicinal ingredients（CHMIs）
cellular infectivity of virus
antiviral activity
