摘要
以携带逆境诱导转录因子AtDREB1A基因的地被菊‘秋艳’转化株系‘1805’为母本,以观赏性状好、应用广的品种‘亚冬之光’为父本,人工授粉杂交,获得杂交后代158株。PCR和RT-PCR检测结果表明,外源AtDREB1A基因能够在有性繁殖过程中稳定地遗传给后代,且在低温胁迫下能在杂交后代植株中增强表达。与非转基因对照植株相比较,携带AtDREB1A基因的杂交后代植株在低温环境下的脚芽扦插繁殖能力显著增强,在冷冻条件下的存活率明显增高。生理指标分析结果表明,在低温环境下,携带AtDREB1A基因的杂交后代植株的脯氨酸含量和超氧化物歧化酶(SOD)活性比对照植株明显增高。
Using the transgenic plant＇Fall Color＇integrated AtDREB1A gene as the female parent,and the＇Asia Winter Light＇,which has the better phenotype and wider application,as the male parent.Through the cross breeding method of non-emasculation artificial pollination,we obtained 158 filial generations.PCR and RT-PCR analysis of the part of progenies with superior ornamental mutation demonstrates that the exogenous gene can be transferred to the filial generations by sexual propagation and the AtDREB1A gene is over-expression in transgenic hybrids.Under low temperature,compared with the wild type,the growing of cuttage propogation plantlets of the transgenic hybrids which carrying exogenous DREB1A gene are much better,and the survival rates obviously increase under freezing stress.Physiological analysis demonstrates that the proline content and SOD activity of the transgenic hybrids are obviously raised under low temperature.
出处
《园艺学报》
CAS
CSCD
北大核心
2011年第9期1717-1726,共10页
Acta Horticulturae Sinica
基金
国家公益性行业(农业)科研专项(200903020)
北京市园林绿化局育种专项(Y1HH201000103)
关键词
菊花
AtDREB1A基因
常规杂交
耐寒性
生理分析
chrysanthemum
AtDREB1A gene
cross breeding
low temperature resistance
biophysical analysis
