摘要
目的回顾HBV-DNA乙肝患者的检测结果并对其进行观察与分析。方法 HBV-DNA试剂由中山大学达安基因股份有限公司提供,采用核酸扩增(PCR)荧光定量检测技术,操作及结果判断均严格按试剂说明书进行。结果表1结果显示大三阳模式中HBV-DNA的阳性检出率明显高于小三阳模式,两者存在明显差异(P<0.01),证明了HBeAg阳性表明病毒复制活跃,传染性强。表2结果说明慢性乙型肝炎HBV感染者肝功能损害程度与其病毒含量有明显的相关性。结论综上所述,通过运用PCR技术检测血中的HBV-DNA初步将结果作出了分析,为临床诊断以及流行病学调查提供了重要依据。
Objective To review the HBV-DNA hepatitis patients test results were observed and analyzed.Methods HBV-DNA reagent from the University of Zhongshan,David Gene Company Limited,the introduction of nucleic acid amplification（PCR） fluorescent quantitative detection technology,operation and result judgement are strictly according to reagent specification.Results The results in Table 1 show big Sanyang mode of HBV-DNA positive rate was significantly higher than that of small Sanyang mode,both have obvious difference（P0.01）,HBeAg proves positive indicating active viral replication and infectivity is strong.Table 2 results show that chronic hepatitis HBV infection impairment of liver function and virus contents had significant correlation.Conclusion To sum up,through the use of PCR technique for the detection of serum HBV-DNA preliminary results to make analysis for the clinical diagnosis and epidemiological investigation provided important basis.
出处
《中外医疗》
2011年第26期8-8,10,共2页
China & Foreign Medical Treatment
