摘要
目的探讨慢性乙型肝炎患者外周血CD4^＋CD25^＋调节性T细胞（CD4^＋CD25^＋ Treg）检测的临床意义。方法慢性HBV感染者48例（慢性HBV携带者10例为慢性HBV携带者组、慢性乙型肝炎18例慢性乙型肝炎组和乙肝肝硬化为乙肝肝硬化组20例），以流式细胞仪检测血CD4^＋CD25^＋ Treg细胞频率，实时荧光定量聚合酶链反应检测血HBVDNA载量。结果血CD4^＋CD25^＋ Treg细胞百分率：慢性HBV携带者组为（6．72±2．60）％、慢性乙型肝炎组为（8．56±3．12）％、乙肝肝硬化组为（11．59±4．34）％，组间差异有统计学意义（均P〈0．05）。血HBVDNA载量：慢性HBV携带者组为1．7×10^6拷贝／ml、慢性乙型肝炎组为4．3×10^5拷贝／ml、乙肝肝硬化组为6．8×10^4拷贝／ml，组间差异有统计学意义（P〈0．01）；慢性肝病患者血CD4^＋CD25^＋调节性T细胞百分率与HBV DNA滴度正相关。结论慢性肝病患者血Treg细胞频率与HBVDNA滴度正相关，提示Treg细胞在慢性乙型肝炎致病机制中起重要作用。
Objective To investigate the clinicai value of circulating T regulatory cell （Treg） in patients with chronic hepatitis B （CHB）. Methods The circulating Treg frequency and the levels of HBV replication in 48 patients with CHB, including 10 HBV carriers, 18 CHB, and 20 liver cirrhosis （LC）, were analyzed by fluorescence-activated cell sorting （FACS） and by real-time Polymerase Chain Reaction （ PCR）, respectively. Results Tregs frequency was （ 11.59 ± 4.34 ） % （ P 〈 0.05 ） in LC and significantly higher than that in CHB （ 8.56 ± 3.12） % or in HBV carries （6.72 ± 2.60） %. HBV DNA copies was 1.7 ×10^6 copies/ml （P 〈 0.01 ） in HBV cartiers and significantly higher than that in CHB （4.3×10^5 ） copies/ml or in LC （6.8 ×10^4 ） copies/ml. Significant relationship was found between Treg frequency and HBV DNA copies. Conclusions Circulating Treg would play an important role in CHB infection with correlated closely with the viral replication.
出处
《中国医药》
2011年第10期1198-1200,共3页
China Medicine
