摘要
研究了辣椒(Capsicum annuum L.)黄绿苗胞质雄性不育系(YBM-A6)、保持系(YBM-B6)及绿苗(96-140)的叶绿素变化及叶片酶活性。从幼苗期到成株期三者的叶绿素含量呈逐步增加的变化趋势,绿苗叶片叶绿素、类胡萝卜含量都远高于黄绿苗,但黄绿苗叶片叶绿素a/b、类胡萝卜素/叶绿素(a+b)(除结果期外)的比值都远大于绿苗。黄绿苗叶片中游离脯氨酸含量均高于绿苗,但其含量变化趋势相同;黄绿苗叶片中过氧化物酶、过氧化氢酶活性变化趋势与绿苗相同;YBM-A6叶片过氧化氢酶活性均低于其保持系YBM-B6,过氧化物酶活性则高于其保持系,超氧化物歧化酶活性在开花结果盛期明显高于保持系YBM-B6和96-140。说明叶绿素和类胡萝卜素含量和变化与辣椒黄绿苗的叶色变化有关,酶活性变化可能与黄绿苗胞质雄性不育系YBM-A6的不育有关。
A comparison of chlorophyll in leaves of the cytoplasmic male sterile pepper（Capsicum annuum L. ） line YBM-A6 with yellow-green seedling marker,maintainer YBM-B6 and 96-140 normal green plant at different developmental stages were conducted in this study. The results showed that from seedling to adult stage YBM-A6, YBM-B6 and 96-140 showed gradual increase of chlorophyll. In 96-140 leaves Chl （a＋b）, carotenoid concentrations are much higher than that of the YBM-A6. However, Chl a/b, Caro/Chl（a＋b） in YBM-A6, YBM-B6 leaf are much greater than in 96-140 leaf. Proline content in YBM-A6 and YBM-B6 leaves was higher than that of 96-140,and the content change pattern was the same in all lines tested. CAT activity of YBM-A6 leaf was lower than that of YBM- B6, POD activity of YBM-A6 leaf was higher than that of YBM-B6 ,SOD activity of YBM-A6 leaf was significantly higher than that of YBM-B6 and 96-140 leaf SOD at the blossom stage. The change of enzyme activity may be associated with sterility of the cytoplasmic male sterile line YBM-A6 with yel- low-green seedling. Our result suggested that the variation of chlorophyll and carotenoid content contribute to the pepper yellow-green leaves color change.
出处
《中国瓜菜》
CAS
2011年第5期20-24,共5页
China Cucurbits And Vegetables
基金
江苏省镇江市农业科技支撑项目(NY2010015)
江苏省重大科技支撑与自主创新项目(BE2008377)
关键词
辣椒
黄绿苗
胞质雄性不育系
叶绿素
酶
Capsicum annuum L.
Yellow-green seedlings
Cytoplasmic male sterile line
Chlorophyll
Enzyme
