摘要
目的探索同型半胱氨酸诱导血管平滑肌细胞凋亡的途径。方法组织贴块法培养血管平滑肌细胞,免疫细胞化学Envision二步法和western blot对平滑肌细胞Fas、caspase-8蛋白的表达进行观察。结果在胞膜和胞浆中可见到Fas表达的阳性产物,而caspase-8蛋白则在胞浆和胞核中见到阳性表达产物,同型半胱氨酸使血管平滑肌细胞Fas、caspase-8蛋白的表达呈浓度和时间依赖性上调(P<0.05)。western blot方法的检测结果与上述结果相符。结论同型半胱氨酸诱导人血管平滑肌细胞凋亡受体Fas表达增加,Fas可介导凋亡启动子caspase-8表达增加,这可能是同型半胱氨酸诱导血管平滑肌细胞凋亡的途径之一。
Objective To explore the apoptosis pathway of the human vascular smooth muscle cells（HVSMCs）induced by homocysteine.Methods HVSMCs were cultured in vitro.The expressions of Fas and caspase-8 protein in HVSMCs were detected by immunohistochemistry Envision two-step method and western blot.Results Immunohistochemistry of Fas protein indicate that the positive particles existed in the both membrane and cytoplasm after the treatment of Hcy.The positive particles of caspase-8 protein existed in the both cytoplasm and nucleus.The intensity of the two proteins increased in time and concentrations dependent manner（P0.05）.Conclusion Homocysteine can increase the expressions of death receptors Fas in HVSMCs.Fas induce the increasing of caspase-8 protein.Death receptors-mediated signaling pathways may be one of the pathway in VSMCs apoptosis induced by homocysteine.
出处
《滨州医学院学报》
2011年第4期270-273,276,共5页
Journal of Binzhou Medical University
