摘要
目的研究α干扰素-1b(IFN-α-1b)对人肝癌细胞系HepG2细胞MMP-2和TIMP-2表达及其表达比例的影响,探讨IFN-α抗肝癌细胞生长与转移复发的相关分子机制。方法培养HepG2细胞,在不同剂量的IFN-α-1b干预下,于不同时间提取上清液,通过ELISA方法检测MMP-2、TIMP-2蛋白的表达浓度。结果在培养HepG2细胞24及48 h后,IFN-α剂量为3000 IU.ml-1时的MMP-2蛋白浓度与空白对照组相比明显降低,分别为(1 725.3±300.7)、(2 899.8±343.2)pg.ml-1,差别均具有统计学意义(P<0.05);各时间段TIMP-2浓度与对照组比较均无显著性差异;MMP-2、TIMP-2浓度比值较对照组显著降低,具有统计学意义(P<0.05)。结论大剂量的IFN-α-1b可抑制HepG2细胞MMP-2的表达水平,下调MMP-2、TIMP-2浓度比值,并呈剂量效应关系。IFN-α对HepG2细胞MMP-2表达的抑制作用在其抗肝细胞癌生长与转移复发中发挥一定作用。
Objective To investigate the effects of IFN-α-1b on the expression of MMP-2 and TIMP-2 protein and the ratio of MMP-2/TIMP-2 in human hepatocellular carcinoma（HCC） cell line HepG2 cells and explore its molecular mechanism of inhibiting the growth and metastasis of HCC.Methods MMP-2,TIMP-2 concentration were measured in the supernate of HepG2 cells incubated in DMEM or exposed to differing dosages of IFN-α（30,300 or 3000 IU.ml-1） by ELISA.Results MMP-2 concentrations in the HepG2 cells incubated for 24 and 48 h exposure to IFN-α 3 000 IU.ml-1 were（1 725.3±300.7） and（2 899.8±343.2） pg.ml-1.There were significant differences compared with controls（P0.05）,whereas no significant differences were found between TIMP-2 concentration of intervention groups and control groups.But there were significant differences between MMP-2/TIMP-2 ratio after incubation for 24 or 48 h.Conclusion IFN-α-1b treatment significantly decreases MMP-2/TIMP-2 ratio by inhibiting the expression of MMP-2 in HepG2 cells in a dose-dependent manner.The down-regulation of MMP-2 levels contributes,at least in part,to its activity of inhibiting the growth and metastasis of HCC.
出处
《滨州医学院学报》
2011年第4期267-269,共3页
Journal of Binzhou Medical University
基金
滨州医学院科研启动基金(BY2005KYQD12)
