摘要
目的观察急性心肌梗死患者血清中微小RNA(miRNA)水平的变化。方法采用3'端加多聚poly(A)尾的反转录后荧光定量PCR的方法测定17例急性心肌梗死患者和11例健康对照者血清中miR-1的水平,并测定其血清肌酸激酶(CK)、肌酸激酶同工酶MB(CK-MB)及心肌肌钙蛋白T(cTnT)水平。结果心梗组miR-1水平较对照组升高(P<0.01);心梗组患者血清miR-1较对照组均值升高的倍数与其血清CK-MB较对照组均值升高的倍数存在正相关关系(r=0.52,P<0.05)。结论 miR-1有可能作为急性心肌梗死的标志物,miR-1水平升高的机制可能与心肌梗死灶周围的缺血组织释放增加有关。
Objective To evaluate the potential application of serum microRNA-1（miR-1） as a diagnostic marker in acute myocardial infarction（AMI）.Methods The serum levels of miR-1 in 17 AMI patients and 11 health control subjects were determined by using the method of poly（A） tailing-based SYBR quantitative PCR,The serum levels of creatine kinase（CK）,creatine kinase-MB（CK-MB） and cardiac troponin t（cTnT） were also determined.Results The level of serum miR-1 was significantly increased in patients with AMI;The multiple of miR-1 level in AMI group/mean value of control group had a positive correlation with the multiple of serum CK-MB level in AMI group/mean value control group.Conclusions The results suggest that serum miR-1 could be a potential diagnostic biomarker for AMI.The mechanism of elevated miR-1 may be associated with increased release of ischemic tissues around the infarct.
出处
《山东医药》
CAS
北大核心
2011年第36期14-16,118,共4页
Shandong Medical Journal
关键词
心肌梗塞
血清
微小RNA
acute myocardial infarction
serum
microRNA-1
