摘要
将来自茶树的冷诱导基因CsCOR1与pGEX-6p-1质粒上的谷胱甘肽-S-转移酶(GST)融合,构建为融合表达载体,最终实现了在0.8 mmol/L IPTG诱导下的原核表达,获得了36 kD的可溶性融合蛋白,为对CsCOR1基因功能的进一步研究奠定基础。
In this paper,CsCOR1 gene as a cold-induced gene from Camellia sinensis,was fused behind the GST gene in pGEX-6p-1,forming a fusion expression vector.Finally,GST-CsCOR1 was induced by 0.8 mmol/L IPTG and a 36 kD fusion protein was synthesized.The present study could provide the fundament for the further functional characterization of CsCOR1.
出处
《生物技术通报》
CAS
CSCD
北大核心
2011年第8期115-117,122,共4页
Biotechnology Bulletin
基金
河南省重点科技攻关项目(082102150009)
信阳师范学院博士启动基金
关键词
茶树
融合基因
原核表达
Camellia sinensis Fusion gene Prokaryotic expression
