摘要
从玉米抗旱自交系CN165中克隆得到了与逆境胁迫相关的bZIP(Basic Leucine Zipper Protein)基因ZmbZIP71。Zm-bZIP71基因的开放阅读框为471bp,编码156个氨基酸,相对分子量为17.59 kDa,等电点pI为9.24。ZmbZIP71蛋白包含真核生物中高度保守的bZIP结构域。ZmbZIP71基因编码区的基因组包括2个外显子和1个内含子。利用实时荧光定量PCR方法分析ZmbZIP71基因在玉米不同组织中的表达差异及其在非生物胁迫下的表达模式,结果表明,该基因在玉米的多个组织和器官中均有表达,其中在雄穗和雌穗中的表达量较高。另外,该基因受干旱、低温和ABA的胁迫诱导上调表达,受盐胁迫下调表达。因此推测ZmbZIP71基因可能在玉米的逆境胁迫响应过程中具有重要作用。
A bZIP （Basic Leucine Zipper Protein） gene, ZmbZIP71, was isolated from maize inbred line CN165. Sequence analysis showed that ZmbZIP71 included a 471bp open reading frame （ORF） encoding a polypeptide of 156 amino acids with predicted molecular mass of 17.59 kDa and pI of 9.24. The deduced protein of ZmbZIP71 was predicted to contain the bZIP structural domain,which was highly conserved in eukaryotes. The ge- nomic DNA of ZmbZIP71 was composed of two exons and one intron in its coding region. The results of real-time PCR analysis indicated that ZmbZIP71 was up-regulated by drought, cold （4~C） and ABA, and down-regulated by NaC1 treatment in seedlings. The tissue-specific expression analysis suggested that ZmbZIP71 was constitutively expressed in maize different tissues and expressed much higher in tassels and female ear than that in other organs. These results suggested that ZmbZIP71 might function as an early stress-responsive gene.
出处
《植物遗传资源学报》
CAS
CSCD
北大核心
2011年第5期775-781,共7页
Journal of Plant Genetic Resources
基金
国家重点基础研究发展计划(973计划)项目(2011CB100100)
国家自然科学基金重点项目(30730063)
