摘要
目的:了解MTHFR基因677和1298位点多态性与宫颈癌发病的关系。方法:应用PCR扩增技术,其扩增产物经限制性核酸内切酶HinfI和MboⅡ酶消化后进行琼脂糖凝胶电泳,分析MTHFR基因型的分布情况。结果:宫颈癌患者157例,CIN患者38例,异常对照组183例,正常对照组199例,发现MTHFR基因的677位点多态性分布差异并无统计学意义(P>0.05),1 298位点多态性的分布在各组间差异有统计学意义(P<0.05)。结论:由于地理环境、种族和生活条件的不同,相同的基因突变在不同的人群中所起的作用可能不同。
Objective：To understand the relationship between polymorphisms of 677 and 1 298 loci in MTHFR gene and the pathogenesis of cervical cancer. Methods：PCR amplification technology was used in the study,the products were digested by restriction endonucleases（HinfI and Mbo Ⅱ）,then agarosegel electrophoresis was carried out to analyze the distribution of MTHFR genotypes. Results：157 patients with cervical cancer,38 patients with CIN,183 patients in abnormal control group and 199 cases in normal control group were included in the study,there was no significant difference in the distribution of polymorphism of 677 locus in MTHFR gene（P〉0.05）,but there was significant difference in the distribution of polymorphism of 1 298 locus in MTHFR gene among different groups（P〈0.05）. Conclusion：The same gene mutation may play different roles in different populations because of the differences of geographic environment,ethnic lines and living conditions.
出处
《中国妇幼保健》
CAS
北大核心
2011年第26期4087-4089,共3页
Maternal and Child Health Care of China
基金
福建省厦门市重大疾病研究项目〔XMWZK0602〕
关键词
MTHFR
基因多态性
宫颈癌
叶酸
MTHFR； Gene polymorphism； Cervical cancer； Folic acid
