摘要
目的探讨增强子结合蛋白C／EBP仅在白血病裸鼠体内的肿瘤抑制作用。方法将30只BALB／C裸鼠随机分转染组（10只）、空载组（10只）、对照组（10只），建立皮下瘤模型，并将30只BALB／c裸鼠如上分组建立白血病模型，将C／EBP仅稳定表达细胞株pEGFP-C／EBPα-K562、空载细胞株pEGFP-K562及白血病细胞株K562分别经皮下和尾静脉注射到相应组裸鼠体内，形成皮下瘤和血液病模型。观测皮下肿瘤的变化，应用TUNEL检测细胞的凋亡，瑞特-吉姆萨染色观察血液病模型裸鼠外周血和骨髓中自血病细胞增殖能力，RT-PCR检测增殖相关基因的表达。结果皮下瘤模型中pEGFP—C／EBPα-K562组肿瘤质量及最大直径为（2．4±0．1）g和（11±2）mm，空载组和对照组分别为（5．1±0.3）g、（19±3）mm和（5．7±0．4）g、（23±3）mm（均P〈0．05），TUNEL检测发现pEGFP-C／EBPα-K562组肿瘤细胞凋亡明显增加（P〈0．05）；血液病模型鼠外周血可见白血病细胞，pEGFP—C／EBPα-K562组白血病细胞增殖能力明显低于空载组和对照组，可见明显细胞分化现象，RT—PCR检测发现p53基因上调和c-myc基因下调。结论增强子结合蛋白C／EBPα在白血病小鼠体内具有促进肿瘤细胞凋亡和抑制白血病细胞增殖的能力，并能促进白血病细胞分化。C／EBPα的白血病抑制作用可能是通过对相应基因的调控来实现。
Objective To explore the role of tumor inhibition of enhancer binding protein C/EBPα in the leukemic mice. Methods BALB/c nude mice were randomly divided into three groups. Three kinds of cells including pEGFP-C/EBPα-K562 cells, pEGFP-K562 cells and K562 cells as the control were injected into mice separately through the subcutaneous and tail vein, and subcutaneous tumors and leukemic models were formed. The changes of tumors were observed and the apoptosis of cells was detected by TUNEL; The capacity of proliferation of leukemia cells was observed in the bone marrow and the peripheral blood by Wright-Giemsa staining. The expression of genes of related to proliferation was detected by RT-PCR. Results The quality and the max diameter of tumors in the pEGFP-C/EBPα-K562 group were smaller than that of pEGFP-K562 group and K562 control group [（2.4±0.1） g vs （5.1±0.3） g and （5.7±0.4） g, both P 〈0.05; （11±2） mm vs （19±3） mm and （23±3） mm, both P 〈0.05]. More apoptosis cells were found in the pEGFP-C/EBPα-K562 group leukemic cells were found in the peripheral blood of leukemic models, and the proliferation of leukemic cells in the pEGFP-C/EBPα-K562 group were lower than that of other groups, accompany by the conspicuous cell differentiation, p53 was significantly elevated by RT-PCR, while down-regulated of c-myc. Conclusion Enhancer binding protein C/EBPα promote the apoptosis of cells and inhibit the proliferation of leukemia cells in leukemia mice, and further induce the cell differentiation. The inhibition of enhancer binding protein C/EBPα in the leukemia may have effect through the regulation of related genes.
出处
《白血病．淋巴瘤》
CAS
2011年第8期475-479,共5页
Journal of Leukemia & Lymphoma
