摘要
目的:探讨康复新液对恶唑酮诱导的小鼠实验性结肠炎的影响及其机制。方法:健康昆明小鼠60只分为5组:正常对照组、模型对照组、康复新液A组、康复新液B组、康复新液C组。除正常组外,其余小鼠均以恶唑酮灌肠造模并给予不同形式的药物干预。造模5d后处死,进行疾病活动指数(DAI)、大体和组织学损伤评分,提取肠黏膜固有层单个核细胞(LPMC),以荧光定量聚酶链反应(PCR)检测表皮生长因子(EGF)、激活蛋白1(AP-1)、核因子κB(NF-κB)、白细胞介素4(IL-4)的表达量。结果:(1)模型组、康复新液A组的DAI、大体和组织学损伤评分均显著高于正常组;康复新液B组则显著低于模型组而更接近于正常组;康复新液C组高于正常组但低于模型组(P<0.05);(2)LPMC中模型组和康复新液A组的EGF表达量显著低于正常组;而AP-1、NF-κB、IL-4的表达量均显著高于正常组。康复新液B组EGF的表达量与正常组无显著差异,而AP-1、NF-κB、IL-4的表达量显著低于模型组而接近正常组。康复新液C组EGF的表达量低于正常组,而AP-1、NF-κB、IL-4的表达量低于模型组而高于正常组。结论:康复新液具有促进EGF表达以修复黏膜的作用,且可通过抑制AP-1、NF-κB、IL-4等炎性递质的表达来达到全身及局部的抗炎作用。
Objective：To investigate the therapeutic effect of Kangfuxin on oxazolone induced colitis in mice.Methods： Sixty mice were randomized into five groups（n=12）： normal group,model group,Kangfuxin A group,Kangfuxin B group and Kangfuxin C group.Except the mice in normal group,all mice were induced by oxazolone enema.The mice in three Kangfuxin groups were treated by Kangfuxin in different ways.Disease activity index（DAI） was assessed during the experiment.All mice were sacrificed 5 days after enema and general inspection and microscopic colonic damage were assessed.Lamina propria mononuclear cells（LPMC） were isolated from freshly tissue.The expression of epidermal growth factor（EGF）,activator protein-1（AP-1）,nuclear factor κB（NF-κB）,interleukin-4（IL-4） in LPMC were detected by fluorescence quantitative PCR（FQ-PCR）.Results： Compared with normal control group,DAI,general inspection and microscopic colonic damage score,as well as the expressions of AP-1,NF-κB,IL-4 mRNA in LPMC increased significantly in model group（P0.05）.The above-mentioned indices in Kangfuxin B group were significantly lower than those in model group（P0.05） and similar to those in normal control group.The expression of EGF in Kangfuxin B group was similar to that in normal control group and higher than that in model group（P0.05）.All indices in Kangfuxin A group were similar to those in model group.Almost all indices in Kangfuxin C group were between those in normal control group and model group.Conclusions： The results indicate that Kangfuxin can attenuate the colonic inflammation in mice with experimental colitis via regulating the expression of cytokine.
出处
《中国临床医学》
2011年第4期446-449,共4页
Chinese Journal of Clinical Medicine
基金
上海市卫生局青年基金资助项目(编号:2008Y038)
