摘要
目的:通过分析比较子宫内膜异位症SD大鼠模型不同种植部位异位病灶的生长特点,为后继内异症的发病机制和治疗研究提供一个合适可选的实验动物模型平台。方法:采用自体移植法分别将成熟SD大鼠的子宫内膜片段种植到肠系膜(n=24)和腹壁(n=21)。从造模后第1周开始,每周每组随机取3只大鼠手术观察病灶的生长情况,直至造模后第8周。结果:肠系膜病灶与周围组织均有不同程度的粘连,手术中极易出血,但其病灶的生长周期长,生长高峰期出现在第5周;而腹壁病灶与周围组织很少有粘连,手术视野清晰,病灶易分离测量,但其病灶的生长周期短,生长高峰出现在第2周,2～4周为一个生长稳定期。结论:腹壁种植的SD大鼠内异症实验模型由于其病灶与周围组织界限清楚,生长稳定,可能是研究药物对异位病灶早期抑制作用的合适动物模型;肠系膜模型因其生长周期长,生长高峰出现晚,可能是研究药物对内异症治疗疗效观察的合适模型。
Objective：By comparing and analysis the growth characteristics of ectopic foci of endometriosis（EMS） SD rats model in different planting sites,view to provide a suitable and selectable animal model platform for the success research of pathogenesis and treatment of endometriosis.Methods：Autologous transplantation technique was adopted and the endometrial tissues of adult SD rats were sutured into the superior mesenterium（n=24）and abdominal wall（n=21）,respectively.Then every week,3 rats were captured randomly in each group for a second operation to observe the growth conditions and measure the size of lesions,which lasted from the 1st week till the 8th week after the first operation.Results：Compared with the abdominal wall lesions,the mesenteric lesions showed different degrees of adhesion with the surrounding tissues,bleeding easily when operated,but the growth cycle was longer and the size peak was in the 5th weak.While the abdominal wall lesions existed rare adhesion with the surrounding tissue,with a clear operative field,and the lesions could be measured and separated easily.The size peak appeared in the 2nd week and can last till the 4th week.But the disadvantage was that it has a shorter growth cycle.Conclusion：Because of its clear boundaries with surrounding tissues and growing stability,the abdominal wall ectopic model of endometriosis maybe a suitable animal model for the drug inhibition of ectopic lesion in the early stage.While the mesenteric model has a longer growth cycle,and the growth peak presented later,maybe it is more suitable for the detection of drug efficacy to the EMs treatment.
出处
《现代妇产科进展》
CSCD
北大核心
2011年第8期628-631,共4页
Progress in Obstetrics and Gynecology
基金
国家自然科学基金资助项目(No:NSFC81070468)
