摘要
目的:通过观察田蓟苷对TNF-α诱导的大鼠主动脉血管平滑肌细胞(VSMC)增殖和迁移的影响,探讨其治疗动脉粥样硬化的可能药效机制。方法:体外培养大鼠主动脉VSMC,采用SABC-Cy3荧光免疫组化技术鉴定其纯度;以TNF-α刺激VSMC建立细胞增殖和迁移模型;采用MTT法检测细胞的增殖,Transwell小室法检测细胞的迁移,免疫酶组化法检测细胞内增殖细胞核抗原(PCNA)的表达水平。结果:2.5～10μg/ml田蓟苷可不同程度抑制TNF-α诱导的VSMC增殖、迁移以及细胞内PCNA的表达,且呈现一定剂量依赖关系的趋势。结论:田蓟苷通过抑制TNF-α对VSMC的增殖和迁移作用,可能是其治疗动脉粥样硬化的药效机制之一。
Objective： To explore the possible mechanism of pharmacological activity of tilianin by observing the effects of tilianin on proliferation and migration of rat vascular smooth muscle cells（VSMCs） induced by TNF-α.Methods： The rat VSMCs were cultured in vitro,and the purity of cells was identified by SABC-Cy3 immunofluroescence technique.Cell proliferation and migration model was established by stimulation with TNF-α.MTT assay was used to evaluate the proliferation of cells,and Transwell chambers were used to detect the migration of cells.The expression of intracellular proliferating cell nuclear antigen（PCNA） were measured by immunoenzymatic histochemistry.Results： The tilianin with different concentrations could inhibit,in varying degrees,the proliferation,migration and expression of intracellular PCNA of VSMCs induced by TNF-α,which showed a tendency toward a dose-dependent manner.Conclusion： The tilianin can inhibit the proliferation and migration of VSMC induced by TNF-α,which may be one of pharmacological mechanisms of tilianin in treatment for atherosclerosis.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2011年第4期17-20,共4页
Pharmacology and Clinics of Chinese Materia Medica
基金
国家科技重大专项课题新药创制(2009ZX09103-448)课题资助
