摘要
目的:探讨曲古抑菌素A(trichostatin A,TSA)对耐伊马替尼细胞株K562R的增殖和凋亡的影响。方法:采用3-(4,5-二甲基-2-基)-5(3-羧基甲基苯基)-2-(4-磺酸苯基)-2H-四氮唑细胞毒试验法观察细胞增殖抑制,细胞凋亡率采用流式细胞仪凋亡试剂盒检测。结果:TSA单用能抑制K562R细胞的增殖,呈时间剂量依赖性,并能促进凋亡。与伊马替尼联用有明显协同作用。结论:TSA与伊马替尼联用具有明显抑制耐伊马替尼的K562R增殖和诱导凋亡的作用,可能为临床伊马替尼耐药的患者提供新的治疗药物。
Objective To explore the effect of trichostatin A （TSA） alone and combination with imatinib on the proliferation and apoptosis of K562R cells.Methods 3-（4,5-dimethylthiazol-2-yl） -5（3-carboxymethoxyphenyl）-2-（4-sulfophenyl）-2H-tetrazolium（MTS） assay was used to observe the proliferation of K562R cells and apoptosis was analyzed by annexin-Ⅴ/propidium iodide（PI） staining.Results After exposure to TSA,the proliferation of K562R cells was inhibited,and the effect was in both time- and dose-dependent manner.The apoptosis was induced.Combined with imatinib,the effect of proliferation inhibition was better.Conclusion TSA combined with imatinib can inhibit the proliferation of K562R cells and induce its apopotosis.TSA may become a new drug for imatinib-resistant patients.
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2011年第8期782-785,共4页
Journal of Central South University ：Medical Science
关键词
曲古抑菌素A
伊马替尼
耐药
增殖
凋亡
trichostatin
imatinb
drug-resistant
proliferation
apoptosis
