摘要
[目的]采用HPLC-DAD比较蓬子菜不同药用部位中芦丁、绿原酸的含量。[方法]用HPLC-DAD梯度洗脱。比较蓬子菜根、茎、叶、花中芦丁和绿原酸含量。[结果]芦丁、绿原酸质量浓度与峰面积呈良好线性关系,线性范围分别是：芦丁0.024 3-1.950 0μg,绿原酸0.132 5-10.600 0μg;芦丁回收率101.02%,绿原酸回收率101.65%;蓬子菜中芦丁和绿原酸的含量花中最高,根中最低。[结论]HPLC-DAD法分离度好、稳定,可作为蓬子菜的质量控制方法;芦丁和绿原酸的含量因药用部位的不同存在差异。
[Objective] To compare the content of chlorogenic acid and rutin in the different parts of Galium verum L.by using the HPLC-DAD method.[Method] A HPLC-DAD method was developed for the content determination of rutin and chlorogenic acid in root,stem,leafage and flower from Galium verum L..[Result] A good linearity was obtained,the linear range were： rutin 0.024 3-1.950 0 μg,chlorogenic acid 0.132 5-10.600 0 μg;the average recovery of rutin was 101.02% and the average recovery of chlorogenic acid was 101.65%.The content of rutin and chlorogenic acid was the highest in flowers and the lowest in roots.[Conclusion] The HPLC-DAD method separation is good,stable and can be used for the quality control of Galium verum L.,the contents of rutin and chlorogenic acid are different in the different parts of Galium verum L..
出处
《安徽农业科学》
CAS
北大核心
2011年第24期14632-14633,14635,共3页
Journal of Anhui Agricultural Sciences
基金
徐州市科技创新项目(XX10A052)
徐州市科技计划项目(XM09B026)
徐州医学院大学生实践创新训练计划项目(2009)
