摘要
[目的]为了研究春兰适应环境变化的方法。[方法]对自然状态(正常组,CO2浓度(370±50)μl/L)和高CO2浓度(试验组,CO2浓度(700±50)μl/L)春兰叶片的叶绿素及叶绿素蛋白质复合物含量进行研究。[结果]与正常组相比,试验组春兰叶片的叶绿素含量增高,叶绿素a/b降低;叶绿素蛋白质复合物的含量增加,经SDS-聚丙烯酰胺凝胶电泳得到6条叶绿素蛋白质复合物带和1条游离色素带,表明叶绿素蛋白质复合物种类未发生变化。从电泳结果可以看出,叶绿素蛋白质复合物聚合态如LHCⅡ3的量增多,单体态LHCⅡ1和LHCⅡ2的量减少。[结论]叶绿素、叶绿素蛋白质复合物含量的变化是春兰对高CO2浓度的一种适应效应,有利于提高其在光合作用中光能的吸收、传递和转换的效率,并且支持高效的光合碳素同化作用。
[Objective]The research aimed to study the method of Cymbidium goeringil adapting to environmental change.[Method] The effects of high CO2 concentration on the chlorophyll and chlorophyll-protein complexes of Cymbidium goeringii leaves were studied.[Result]Compared with the normal CO2 concentration（370±50 μl/L）,the contents of chlorophyll and chlorophyll-protein increased at high CO2 concentration（700±50 μl/L）.The contents of polymer state of PSⅡlight-harvesting chlorophyll a/b-protein complex（LHCⅡ） increased as the monomer state of LHCⅡdecreased.The changes of the contents of chlorophyll and chlorophyll-protein,and the state of LHCⅡin Cymbidium goeringii were an adaptive effects of plant photosynthetic mechanism to the long term elevated CO2.[Conclusion] These changes increased the efficiency of the absorption,transfer and conversion of light energy in plant photosynthesis,and support the high efficiency of photosynthetic carbon assimilation.
出处
《安徽农业科学》
CAS
北大核心
2011年第24期14523-14524,14527,共3页
Journal of Anhui Agricultural Sciences
关键词
高CO2浓度
春兰
叶绿素
叶绿素蛋白质复合物
High CO2 concentration
Cymbidium goeringii Rchb.f.
Chlorophyll
Chlorophyll-protein complexes
