摘要
荧光光谱法研究了注射用核黄素(R iboflavin,R f)与牛血清白蛋白(BSA)的相互作用。核黄素对BSA具有荧光猝灭作用,其猝灭方式为静态猝灭;在301和317K下用Stern-Volm er方程和热力学方程等处理实验数据,求出了结合常数KA、结合位点数n及热力学参数ΔG、ΔH和ΔS。核黄素与BSA之间的作用力主要为氢键和范德华力。Cu2+的加入对核黄素与BSA的结合常数和结合位点数有一定影响。
The interaction between riboflavin and bovine serum albumin（BSA） was studied by fluorescence spectrum.The results indicated that the fluorescence of BSA was quenched by riboflavin,and the combination of them was a static quenching process.The quenching data were analyzed based on Stern-Volmer equation,and the quenching constant,binding constant and binding sites were obtained.After analyzing fluorescence quenching data by Stern-Volmer equation and thermodynamic equation,the value of bonding constant KA,thermodynamic parameters（ΔH,ΔG and ΔS） were obtained.The quenching constant,binding constant and binding sites of the interaction between riboflavin and BSA were influenced in the presence of Cu（Ⅱ）.
出处
《化学试剂》
CAS
CSCD
北大核心
2011年第9期812-815,共4页
Chemical Reagents
基金
山东省教育厅科技计划资助项目(J08LG15)
