摘要
目的:探讨镰形棘豆水提取物(EOF)对脓毒症大鼠血清细胞因子水平的影响。方法:采用盲肠结扎穿孔术(CLP)制备动物脓毒症模型,将雄性SD大鼠随机分为5组,正常对照组(6只)、假手术组(24只)、模型组(24只)、EOF低、高剂量组(0.5,2 g.kg-1,24只),于造模后灌服1次。于造模后2,6,12,24 h取大鼠动脉血,采用ELISA测定血清肿瘤坏死因子-α(TNF-α),白细胞介素-6(IL-6),白细胞介素-1(IL-1)水平。结果:相同时间点模型组TNF-α,IL-6,IL-10的水平均高于假手术组(P<0.01);EOF治疗组与模型组相比(除2 h外),TNF-α,IL-6的水平各时间点降低(P<0.05),IL-10的水平升高(P<0.05);EOF高剂量与EOF低剂量相比(除2 h外),TNF-α,IL-6水平各时间点降低更明显(P<0.05),IL-10的水平各时间点升高更明显(P<0.05)。结论:EOF能下调脓毒症大鼠TNF-α,IL-6的水平,上调IL-10的水平;EOF具有抗炎和免疫调节作用,有进一步开发为临床治疗脓毒症新药的潜在可能性。
Objective：To investigate water extract（EOF） intervention in septic rats,and to explore EOF on serum cytokines in septic rats.Method：The male SD rats were randomly divided into 5 groups：normal control group（n=6）,sham operation group（n=24）,model group（n=24）,EOF low dose（0.5 g·kg-1） group（n=24） and high dose EOF（2 g·kg-1） group（n=24）.Experimental model was established by cecal ligation and puncture（CLP）,2,6,12,24 h after modeling blood was collected and double antibody sandwich enzyme-linked immunosorbent assay（ELISA） was used to determine serum TNF-α,IL-6,IL-10 level.Result：In the model group TNF-α,IL-6 and IL-10 were higher than those in the sham group（P0.01）.EOF treatment group showed（except 2 h）,TNF-α and IL-6 were decresed（P0.05）,and IL-10 inctesed（P0.05）.Compared with those in the model group.High dose EOF showed stronger effects,compared with those in the low dose EOF（P0.05）.Conclusion：EOF can reduce sepsis TNF-α,IL-6 expression;increase expression of IL-10 inrats with sepsis.EOF has anti-inflammatory and immunomodulatory effects.It might become new drug for clinical treatment of sepsis.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第18期214-217,共4页
Chinese Journal of Experimental Traditional Medical Formulae
关键词
藏药镰形棘豆水提取物
脓毒症
细胞因子
water extract of tibetan medicine oxytropis falcata bung
sepsis
cytokine
