摘要
目的调查山西地区血管紧张素原(angiotensinogen,AGT)和α-内收蛋白(alpha-aduction,ADD1)基因多态性与原发性高血压(essential hypertension,EH)的关系。方法采用病例对照研究,用诱变分离聚合酶链反应(mutagenically separated polymerase chain reactions,MS-PCR)检测山西地区EH患者299例(病例组)和血压正常者218例(对照组)的AGT基因M235T多态性和ADD1基因Gly460Trp多态性。结果病例组AGT基因的TT基因型分布与对照组差异有统计学意义(P<0.001),但病例组T等位基因频率(0.426)与对照组(0.378)相比,差异无统计学意义(P=0.121)。病例组ADD1基因型分布和等位基因频率与对照组相比,差异均无统计学意义(P分别为0.306和0.072)。TT+TT联合基因型患EH的危险度增加(MT+GG:OR=0.410,P=0.041;MT+GT:OR=0.364,P=0.020;MT+TT:OR=0.262,P=0.002)。Logistic回归分析提示,AGT基因的TT基因型较MM、MT基因型患EH的相对危险度增加(MM:OR=0.502,P=0.019;MT:OR=0.306,P<0.001)。结论 AGT M235T多态性与山西地区人群患EH相关,TT基因型可能与EH危险增加有关。AGT M235T多态性与ADD1基因Gly460Trp多态性间存在基因间的协同作用。
Objective This case-control study was designed to investigate the association between angiotensinogen gene polymorphisms and alpha-adducin polymorphisms and essential hypertension （EH）. Methods A case-control study was conducted using performed mutagenically separated polymerase chain reactions （MS-PCR） techniques to explore the AGT gene M235T polymorphisms and the ADD1 gene Gly460Trp polymorphisms in 299 EH and 218 normotensives （NT） of Shanxi Province. Results The genotypes distribution of TT in EH cases was significantly higher than in NH cases （ P〈 0. 001 ）, but not the alletic frequency of T （0. 426 vs. 0. 378, P = 0. 121 ）. No significant difference were seen in the genotype distribution and the alletic frequency of ADD1 gene between EH and NH cases （P = 0. 306, P = 0. 072）. The genotype distribution of TT ＋ TTT was significantly higher to get EH. （ MT ＋ C/G： OR = 0. 410, P = 0. 041 ; MT ＋ GT- OR = 0. 364, P = 0. 020; MT ＋ TT： OR = 0. 262, P = 0. 002 ）. Those who exosed to TT genotype of AGT gene was the easlest to get EH. （MM. OR=0.502, P=0.019;MT：OR=0.306,P〈0. 001）. Conclusions TheAGT M235T polymorphism might be associated with Eli in Shanxi population. TT genotype might be associated with the increased risk of EH. ACT M235T polymorphism might have a gene-gene synergetic effect with Gly460Trp polymorphism.
出处
《中华疾病控制杂志》
CAS
2011年第9期740-744,共5页
Chinese Journal of Disease Control & Prevention
基金
山西省科技攻关项目(20080312018)
关键词
高血压
基因型
病例对照研究
Hypertension
Genotype
Case-control studies
