摘要
背景:近年来脂肪源性基质细胞成为研究与应用的热点之一,但前列腺素 E1 对脂肪源性基质细胞增殖的作用影响尚未见报道。目的:观察前列腺素 E1 对人类脂肪源性基质细胞体外增殖的影响。方法:从人正常脂肪组织分离培养脂肪源性基质细胞,用 5 种不同浓度的前列腺素 E1 处理培养的细胞,其中 3×10-6,6×10-6,9×10-6,12×10-6 mol/L 作为实验组,0×10-6 mol/L 作为对照组,观察经不同浓度前列腺素 E1 处理后细胞的增殖情况。结果与结论:不同浓度实验组的细胞数量与对照组相比均显著升高,差异有显著性意义(P<0.05),各实验组内相比,细胞数量增殖无显著性差异(P>0.05)。提示前列腺素 E1 具有促进脂肪源性基质细胞的增殖作用,且前列腺素 E1 的影响效果无剂量依赖性。
BACKGROUND：Recently,research and application of human adipose derived stromal cells（ADSCs） has become a hotspot;however,there are few reports addressing prostaglandin E1（PGE1） effects on ADSCs proliferation.OBJECTIVE：To explore the effects of PGE1 on ADSCs proliferation.METHODS：ADSCs were obtained from human normal adipose tissue after isolation and culture.The cultured ADSCs were treated with five different concentration of PGE1,which were 3×10-6,6×10-6,9×10-6,12×10-6 mol/L,and 0×10-6 mol/L（control group）.After 3 days,the cell morphology and proliferation were observed.RESULTS AND CONCLUSION：There was no obervious different among the experimental groups.And the cell number of each experimental group was obervious elevated,compared with that of the control group（P0.05）.These findings indicated that ① PGE1 promoted the proliferation of ADSC;② The effect of PGE1 had no dose dependent.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2011年第14期2517-2520,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
