摘要
[目的]探讨补体C3、C4在复发性自然流产(recurrent spontaneous abortion,RSA)发病机制中的作用。[方法]选取30例有RSA史患者为研究组,其中15例未孕者为研究组Ⅰ,15例难免流产者为研究组Ⅱ;30例正常妇女为对照组,其中15例未孕者为对照组Ⅰ,15例正常妊娠者为对照组Ⅱ。采用免疫散射比浊法检测各组血清中补体C3、C4值;采用快捷型免疫组化染色法检测两组妊娠者绒毛中补体C3、C4的表达情况。[结果]①研究组血清补体C3、C4水平为(1070.4±240.8)mg/L和(244.2±85.8)mg/L,对照组分别为(929.9±146.6)mg/L和(193.1±65.1)mg/L,两者之间相比差异有显著性意义(P<0.05);②研究组Ⅱ血清补体C3、C4水平为(1167.4±142.8)mg/L和(250.9±81.9)mg/L,对照组Ⅱ分别为(939.1±120.1)mg/L和(202.8±50.9)mg/L,两者之间相比差异有显著性意义(P<0.05);③在研究组Ⅱ和对照组Ⅱ的绒毛组织中,补体C3表达的阳性强度平均积分为(10.1±4.9)分和(2.2±1.4)分,补体C4表达的阳性强度平均积分为(6.6±5.3)分和(1.7±1.0)分,两者之间差异均有显著性意义(P<0.05)。[结论]RSA患者体内补体C3、C4水平升高,补体过度激活可能是RSA的免疫学发病机制之一。
[Objective] To investigate the effect of complement C3 and C4 in the recurrent spontaneous abortion（RSA）.[Methods] Thirty women who had the history of RSA were involved in study group,in which fifteen of them without pregnancy were in study group I,and other women diagnosed as inevitable abortion were in study group II.Thirty normal women were in control group,according to pregnancy or not,they were divided into control group I and group II.Immunonephelometric assay was used to test complement C3 and C4in the serum of all groups and shortcut immunohistochemistry staining was applied to observe complement C3 and C4 expression in villus tissue of two pregnant groups.[Results] ① The complement C3 and C4 were（1070.4±240.8） mg/L and（244.2±85.8） mg/L in study group serum,and were（929.9±146.6） mg/L and（193.1±65.1） mg/L in the control group（P〈0.05）.② The complement C3 and C4 were（1167.4±142.8） mg/L and（250.9±81.9） mg/L in study group II serum,and were（939.1±120.1） mg/L and（202.8±50.9） mg/L in the control group II（P〈0.05）.③ In villus tissue of study group Ⅱ and control group II,the average points positive intensity of complement C3 were（10.1±4.9） points and（2.2±1.4） points,C4 were（6.6±5.3） points and（1.7±1.0） points（P〈0.05）.[Conclusions] The complement C3 and C4 are increased in patients of RSA.Its activation may be one of immune pathogenesis in RSA.
出处
《大连医科大学学报》
CAS
2011年第4期353-356,365,共5页
Journal of Dalian Medical University
