摘要
为了解过渡金属华法灵配合物的抗凝血机理,本文采用荧光光谱、紫外吸收光谱和圆二色谱法(CD)研究了具有抗凝血作用的华法灵过渡金属配合物与人血清白蛋白(HSA)的相互作用。观察到配合物能使人血清白蛋白荧光产生猝灭现象,猝灭方式为静态猝灭,并计算了配合物与人血清白蛋白的结合常数K(约106)和结合位点数n(>1)。根据不同温度下的热力学函数,确定了配合物与人血清白蛋白之间的作用力为氢键和范德华力。发现华法灵过渡金属配合物的存在改变人血清白蛋白的构象,并讨论了配合物使人血清白蛋白构象发生变化的可能原因。配合物的抗凝血作用与其通过改变HSA的构象,进而影响血清白蛋白在血液中的溶解性之间有一定的关系。
For invesitigation the mechanism of the anticoagulant of the complexes of transition metal with warfarin,fluorescence,CD and UV spectra were used to study the interaction between human serum albumin （HSA）and the binary complexes of transition metals with warfarin. It was observed that complexes could reduce the fluorescence intensity of human serum albumin. The way of fluorescence quenching was static quenching, and then calculated the binding constant K （about 106） and binding sites n （〉1）. According to the thermodynamic functions at different temperatures, the binding type of complexes to HSA is hydrongen bond and van der Waals. And found that complexes have obviously changed the conformation of HSA. The possible reason for which the complex made HSA conformation changes was discussed. The interaction between HAS and the complexes warfarin caused the conformation changes the some reasons for anticoagulant function of HSA and influenced the solubility of HSA in blood,which may be of the complexes.
出处
《无机化学学报》
SCIE
CAS
CSCD
北大核心
2011年第9期1772-1780,共9页
Chinese Journal of Inorganic Chemistry
基金
国家自然科学基金(No.20871099)
甘肃省高分子材料重点实验室和兰州理工大学博士科研启动资助项目
