摘要
蜕皮激素20-羟基蜕皮酮(20-hydroxyecdysone,20E)是一种典型的类固醇激素,主导调控昆虫的蜕皮、变态、生殖等重要生理过程。20E受体EcR-USP已被鉴定近20年,20E与其受体复合物的转录调控机制也有了许多重要突破。已有研究表明:(1)20E受体由核受体EcR和USP形成;(2)EcR-USP异源二聚体在分子伴侣蛋白复合物的协助下获得DNA结合活性;(3)20E通过解除共阻遏因子和募集共激活因子来激活EcR-USP异源二聚体并启动下游基因的转录;(4)20E-EcR-USP配体-受体复合物引发20E初级应答基因的表达,由20E初级反应基因编码的转录因子诱导表达的20E次级应答基因级联放大20E信号,从而调控昆虫蜕皮、变态、生殖等生理过程。
The molting hormone,20-hydroxyecdysone（20E） ,is an insect steroid hormone which plays a dominant role in the regulation of insect molting,metamorphosis,reproduction and other physiological processes.The 20E nuclear receptor,EcR-USP,has been identified for 20 years,and several breakthroughs have been recently made in understanding the transcriptional regulation mechanisms of 20E-EcR-USP.Recent research indicates that：（1） the 20E receptor is composed of two nuclear receptor molecules,i.e.,EcR and USP.（2） The EcR-USP heterodimer obtains DNA binding activity with the assistance of a molecular chaperone-containing heterocomplex.（3） 20E transcriptionally activates the EcR-USP heterodimer by releasing co-repressors and recruiting co-activators.（4） The ligand-receptor complex,20E-EcR-USP,triggers a transcriptional cascade,including transcription of the 20E primary-response genes and the 20E secondary-response genes induced by transcription factors encoded by the 20E primary-response genes,to regulate physiological and developmental events.
出处
《昆虫学报》
CAS
CSCD
北大核心
2011年第8期933-937,共5页
Acta Entomologica Sinica
基金
国家自然科学基金项目(30870299,30870335)
上海市重点项目(10JC1416700)
中国科学院“百人计划”择优支持项目
中国科学院知识创新工程项目(KSCX-EW-J-12)
