摘要
[Objective]The aim of this study was to reveal the molecular evolutionary characteristics of dihydroflavonol 4-Reductase（DFR）—a key enzyme in anthocyanin synthesis pathway in Lamiidae plants.[Method]Based on DFR amino acid and cDNA sequences released by NCBI,molecular evolution of DFR gene was analyzed with bioinformatic software.[Result]Seventeen plants fell into two major branches,Lamiidae and Liliidae.Most of the plants within a same family cluster assembled together,which basically accords with the morphological development of species.Prediction results based on selection pressure using MEGA,DNAsp and PAML software showed that DFR gene in Lamiidae plants underwent strong purifying selection pressure.[Conclusion]Our results laid basis for analyzing the molecular mechanism of enzymatic characteristics of DFR and of the anthocyanin synthesis pathway.
[目的]研究唇形亚纲植物中花色素苷合成的关键酶—DFR的分子进化特性。[方法]基于Genbank数据库上发表的唇形亚纲植物DFR氨基酸序列和cDNA序列,利用生物信息学软件对其进行了分子进化分析。[结果]基于DFR氨基酸序列的系统发育树显示,唇形亚纲植物和百合亚纲植物分为2支,大部分同科植物聚在一起,这与形态学上的物种发育关系基本吻合;MEGA、DNAsp和PAML的选择性压力预测结果均显示,DFR基因在唇形亚纲植物进化过程中经历了强烈的纯化选择压力。[结论]该研究可为研究DFR的酶学特性花色素苷生物合成的分子机制等奠定基础。
基金
Supported by the Fundamental Research Funds for the Central Universities(SWJTU09BR208)~~
