摘要
目的探讨检测血清葡萄糖-6磷酸异构酶（GPI）在类风湿关节炎中的诊断价值。方法用双抗体夹心酶联免疫吸附试验ELISA法检测130例RA患者、79例其他自身免疫性疾病患者和54例健康对照者血清中的GPI浓度，并同时检测抗环瓜氨酸肤（CCP）抗体、类风湿因子（RF），RA组还同时检测了C-反应蛋白（CRP）、红细胞沉降率（ESR）以及RA患者的手足X线片检查．结果RA患者血清中GPI浓度显著高于其他风湿病组和健康对照组（P〈O．05），GPI在类风湿关节炎患者中的阳性率为67．1％，特异度为94．7％，抗CCP抗体阳性率为71．5％，RF阳性率为75．4％。130例RA患者GPI阳性率与抗CCP抗体阳性率比较差异无统计学意义（P〉0．05），GPI阳性率与RF阳性率比较差异有统计学意义（P〈0．05），GPI与疼痛关节数和关节肿胀有相关性。结论GPI有可能成为检测RA的又一个独立而敏感的新指标，从而提高RA诊断的敏感性和可靠性，具有较好的临床研究及应用价值。
Objective To probe that the value of glucose-6-phosphate isomerase （GPI） in the diagnosis of rheumatoid arthritis. Methods Test the GPI content in the serum of 130 rheumatoid arthritis （RA） patients, 79 patients with other autoimmune diseases and 54 healthy individuals, and cyclic citrullinated peptide （CCP） antibodies,and rheumatoid factors （RF）. In addition,C-reactive protein （CRP） and erythrocyte sedimentation rate （ESR） were tested,and the X-ray radiog- raphy of RA patients＇ hand and foot was also tested. Results The GPI content in the serum of RA patients was found distinctly higher than other rheumatism patients and healthy individuals （P〈0. 05）,the positive rate of GPI in RA patients was 67.1% and specificity was 94.7% ,the positive rate of anti-CCP antibody was 71.5% ,and the positive rate of RF was 75.4%. The difference comparison between the positive rate of GPI and anti-CCP antibody makes no sense in statistics （P 〉0. 05）,while the difference comparison between the positive rate of GPI and RF makes sense （P〈0. 05）,and GPI was related with the number of painful joint and swelling of joint. Conclusion GPI is possible to be another independent and sensitive new index to raise the sensitivity and reliability of RA diagnosis,and it is valuable for clinical research and application.
出处
《现代检验医学杂志》
CAS
2011年第4期90-91,95,共3页
Journal of Modern Laboratory Medicine
