摘要
目的探讨肿瘤坏死因子相关凋亡诱导配体（TRAIL）和半胱天冬酶-3（Caspase-3）在再生障碍性贫血（AA）骨髓单个核细胞中表达及其在AA造血干细胞凋亡中的作用。方法选取15例初诊和治疗后进入缓解期的AA患者、15例骨髓象正常的患者为研究对象，采用流式细胞术检测患者骨髓单个核细胞中TRAIL表达，采用酶标仪检测骨髓单个核细胞Caspase-3的表达。结果AA初诊时、缓解期和对照组TRAIL蛋白表达分别为（5．94±2．57）％、（2．87±1．72）％和（3．01±2．06）％，Caspase．3蛋白表达分别为（1．3840±0．0236）、（0．8018±0．0126）和（0．7441±0．0112），AA初诊时TRAIL和Caspase-3蛋白表达显著高于缓解期和对照组，差异均有统计学意义（均P〈0．05）；对照组TRAIL和Caspase-3蛋白表达高于AA缓解期，但差异均无统计学意义（均P〉0．05）。结论AA患者骨髓单个核细胞中TRAIL和Caspase-3呈高表达，它们可能参与诱导AA患者造血细胞凋亡。
Objective To study the expression and clinical significance of TNF related apoptosis-inducing ligand（TRAIL） and Caspase-3 in the bone marrow mononuclear cell（BMMNC） of aplastic anemia（AA）, and to approach their effect on the apoptosis of haemopoietic stem cell in AA patients. Methods The TRAIL and Caspase-3 expression in 15 cases with newly diagnosed AA patients and remission patients ,and 15 cases with normal bone mar- row patients were detected by flow cytometry and microplate reader. Results The TRAIL expression of newly diag- nosed AA patient, emission AA patients and control group was （ 5.94 ± 2.57 ） %, （ 2. 87 ±1.72 ） % and （ 3.01 ±2. 06）% respectively, the Caspase-3 expression of newly diagnosed AA patient, emission AA patients and control group was （ 1. 3840 ± 0. 0236）, （ 0. 8018 ± 0. 0126 ） and （ 0. 7441±0：0112 ） respectively. The TRAIL and Caspase-3 expression of newly diagnosed AA patient were significantly higher than the remission AA patients and control group, the difference was significant （all P 〈 0. 05 ） ;The TRAIL and Caspase-3 expression of control group were higher than the remission AA patients, but the difference was not significant （ all P 〉 0.05 ）. Conclusion The TRAIL and Caspase-3 had high expression in the BMMNC of AA patients, and the apoptosis induced by TRAIL and Caspase-3 should play an important role in the pathogenesis of AA.
出处
《中国基层医药》
CAS
2011年第17期2313-2315,共3页
Chinese Journal of Primary Medicine and Pharmacy
