摘要
建立一种高效液相色谱法(high performance liquid chromatography,HPLC)检测食品中人工合成甜味剂安赛蜜的方法。采用C18反相色谱柱,二极管阵列检测器(diode array detector,DAD),以甲醇:0.02mol/L乙酸铵溶液=5.5:94.5(V/V)为流动相,检测波长230nm,流速0.8mL/min,柱温30℃,进样量5μL。安赛蜜出峰时间约在4.6min,样品的检出限为1.7mg/kg,线性范围为1.0～50.0μg/mL,安赛蜜的加标回收率在90%以上,重复性实验的相对标准偏差在2%以下(n=6)。所建立的方法前处理简单,以水为提取溶剂超声波提取安赛蜜,然后在提取液中加入沉淀剂除杂,具有简单、快速、准确、实用性强的特点。
A high performance liquid chromatography method for the determination of the artificial sweetener acesulfame K in food was developed.Ultrasonic-assisted aqueous extraction followed by the removal of protein impurities by adding zinc acetate and potassium ferrocyanide was used for sample preparation.Acesulfame K was separated on a C18 column using methanol-0.02 mol/L ammonium acetate（5.5：94.5,V/V） as mobile phase at a flow rate of 0.8 mL/min and detected using a diode array detector（DAD） at 230 nm.The column temperature was set at 30 ℃ and the injection volume 5μL.The retention time of acesulfame K was about 4.6 min.The detection limit of the developed method was 1.7 mg/kg,and the linear range was between 1.0 and 50.0μg/mL.The mean spike recoveries for acesulfame K in a blank juice sample exceeded 90%,with a relative standard deviation below 2%（n=6）.In this method,acesulfame K was extracted using water as solvent with the assistance of ultrasound,and purified by precipitation.The method proved to be simple,rapid,accurate and practical.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第16期245-248,共4页
Food Science
关键词
安赛蜜
高效液相色谱
测定
acesulfame K
high performance liquid chromatography（HPLC）
determination
