摘要
目的为了解志贺菌毒力大质粒能否在体外转移及其转移后是否仍具有致病性。方法将人源福氏志贺菌及其毒力大质粒分别与鸡源鲍氏志贺菌混合,并在37℃培养箱和4℃冰箱中混合培养24 h;用不同剂量的毒力大质粒转移菌株通过口服和腹腔注射分别人工感染雏鸡和小白鼠。结果人源福氏志贺菌的毒力大质成功转移到丢失自身毒力大质粒的鸡源鲍氏志贺菌中;转移菌株对雏鸡和小白鼠均有致病性,且转移菌株的培养特性也有所改变。结论当志贺菌的毒力大质粒裸露出来且具有适宜的条件时,可以在体外转移到相近的病原菌中,且新生成的转移菌株具有一定稳定性和致病性;志贺菌的高致病性需要有毒力大质粒和菌体的其他特殊致病因子同时存在才能完成。
Objective To understand whether the strong virulent plasmid of Shigella can transfer in vitro and still possess pathogenicity after the transfer. Methods Human Shigella flexneri and the large virulent plasmid of Shigella were separately mixed with chicken Shigella boydii, and then cultured at 37℃ and 4℃ refrigerator for 24hours. Chickens and mice were experimentally infected either orally or intraperitoneally with transferred-strain of large plasmid in different dosages. Results The strong virulent plasmid of human Shigella was successfully transferred into chicken Shigella boydii which had lost the large virulence plasmid. Chicken and mice inoculated with transferred-strain showed pathogenicity of the strains trans- ferred. The culture characteristics of transferred-strain was changed. Conclusion The strong virulent plasmid of Shigella in a suitable environment can be transferred in to pathogenic bacteria in the close category in vitro and the new transfered-strain possessed certain stability and pathogenicity. The high pathogenicity of Shigella would occured if there were strong virulent plasmid and other special causative agents of bacteria simultaneously.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2011年第9期1126-1128,共3页
Chinese Journal of Public Health
基金
国家自然科学基金(30671555
30972187)
关键词
志贺菌
毒力大质粒
质粒体外转移
人工感染
致病性
Shigella
strong virulent plasmid
plasmid transfer
artificial infection
pathogenicity
