摘要
采用单因素实验法,以树脂的饱和吸附量、产品的得率及纯度为指标,考察各因素对动态吸附与解吸的影响,优化大孔树脂动态吸附分离无患子皂苷的工艺条件。结果表明,当pH为4.5的无患子皂苷水提液以流速1 mL/min通过高径比为5.4∶1的吸附柱时,AB-8树脂对无患子皂苷的饱和吸附量达568 mg/g;采用1.5 BV的95%乙醇以1 mL/min的流速洗脱吸附后的树脂,产品得率为83.48%、纯度达93.00%;树脂重复使用8次后其吸附解吸性能基本不变。该方法可以用于无患子皂苷的工业化分离提纯。
To optimize the dynamic adsorption and desorption process parameters for sapindus-saponins onto AB-8 resin,the monofactorial experiments were used to analyze the effects of the process parameters on the saturated adsorption capacity,the recovery yield and the purity of the sapindus-saponins.The results showed that the saturation adsorption capacity of sapindus-saponins on AB-8 resin achieved 568 mg/g when the crude sapindus-saponins water extracts（pH4.5） flowed through the resin column（the ratio of height to diameter 5.4∶ 1） at 1 mL/min.The elution parameters were as follows： 1.5 BV 95% ethanol aqueous solutions at 1 mL/min.The sapindus-saponins content in the final product was 93% with a recovery yield of 83.48%.In addition,the stability of AB-8 resin was fine after eight-time recycled.This method could provide a potential approach for large-scale separation and purification of sapindus-saponins for its wide pharmaceutical use.
出处
《天然产物研究与开发》
CAS
CSCD
2011年第4期709-712,共4页
Natural Product Research and Development
基金
安徽高校省级自然科学研究重点项目(KJ2009A002)
合肥工业大学研究生创新基金项目(XS08042)
关键词
无患子皂苷
AB-8树脂
动态吸附-解吸
吸附量
sapindus-saponins
AB-8 resin
dynamic adsorption-desorption
adsorption capacity
