期刊文献+

一步法实时荧光定量PCR检测大鼠胰腺组织中Insulin mRNA和PDX-1 mRNA的表达 被引量:1

One-Step Real-Time Fluorescent Quantitative PCR Method to Detect the Expression of Insulin mRNA and PDX-1 mRNA in the Pancrease Tissue of Rats
下载PDF
导出
摘要 目的通过检测大鼠胰腺组织中Insulin mRNA和PDX-1 mRNA的表达探讨EGF/Gastrin联用促进胰岛PDX-1表达增强可能的作用机制。方法采用一步法实时荧光定量PCR检测各组大鼠胰腺组织中Insulin基因和PDX-1基因在转录水平的表达。结果大鼠胰腺组织中Insulin mRNA在转录水平的表达情况正常对照组最高;糖尿病组最低,与正常对照组相比相差2.4倍(P<0.05);EGF/Gastrin组是糖尿病组的2.1倍(P<0.05)。大鼠胰腺组织中PDX-1mRNA在转录水平的表达情况正常对照组最高,糖尿病组最低,与正常对照组相比相差2.8倍(P<0.05),EGF/Gastrin组是糖尿病组的2.2倍(P<0.05)。结论 EGF/Gastrin联用促进胰岛PDX-1表达增强的作用机制可能通过其改善胰岛β细胞功能、降低血糖进而减弱糖毒性对PDX-1表达的不良影响,间接地使PDX-1的表达增强。而EGF/Gastrin联用促进胰岛新生、改善胰岛β细胞功能又有可能是通过提高PDX-1的表达而实现的。 Objective To discuss the possible mechanism of the PDX-1 expression enhancement caused by combinated use of EGF and Gastrin through detection of insulin mRNA and PDX-1 mRNA genes expression in the pancrease tissue of rats.Methods Evaluate the expression of insulin gene and PDX-1 gene in the transcriptional level with the One-Step Real-Time Fluorescent Quantitative PCR Method.Results The transcription levels of Insulin mRNA in the Control group was the highest while the DM group was the lowest.The transcription level of Insulin mRNA in the Control group was 2.4 times higher than that in the DM group and the level in the EGF/Gastrin group was 2.1 times higher than the DM group.The transcription levels of PDX-1 mRNA in the Control group was the highest while the DM group was the lowest.The transcription level of PDX-1 mRNA in the Control group was 2.8 times higher than that in the DM group and the level in the EGF/Gastrin group was 2.2 times higher than the DM group.Conclusion The combinated use of EGF and Gastrin can promote the expression of PDX-1 by improving pancrease β-cell functions and reducing blood glucose levels to impair the negative effects of glucose toxicity on PDX-1 expression indirectly.However,the mechanism of promotion on the pancrease cells and improvement of pancrease β-cell functions may be realized by the enhancement of PDX-1 expression.
出处 《中国实验诊断学》 北大核心 2011年第8期1269-1273,共5页 Chinese Journal of Laboratory Diagnosis
基金 吉林省卫生厅科研课题(2009Z052) 吉林省科委课题(200505205)
关键词 大鼠胰腺组织 PDX-1基因 Insulin基因 一步法实时荧光定量PCR pancrease tissue of rats PDX-1 gene Insulin genes One-Step Real-Time Fluorescent Quantitative PCR
  • 引文网络
  • 相关文献

参考文献13

  • 1Winer,J,Jung,C.K,Shackel,I,and Williams,P.M[J].Anal.Biochem,1999,270:41. 被引量:1
  • 2Schmittgen,T.D.,Zakrajsek,B.A.,Mills,A.G.,Gorn,V.,Singer,M.J.,and Reed,M.W.[J].Anal.Biochem,2000,285:194. 被引量:1
  • 3Helena Barbosa,Silvana Bordin,Luiz Stoppiglia,Kelly Silva,Maria Borelli,Héctor Del Zotto,Juan Gagliardino,Antonio Boschero.Islet Neogenesis Associated Protein (INGAP) modulates gene expression in cultured neonatal rat islets[J].ScienceDirect,2006,136:78. 被引量:1
  • 4杨闯..不同纯度大鼠胰岛中CK-19、PDX-1阳性细胞的表达研究[D].重庆医科大学,2006:
  • 5Finegood DT,Scaglia L,Bonner-Weir S.Dynamics of β-cell mass in the growing rat pancreas.Estimation with a simple mathematical model[J].Diabetes,1995,44:249. 被引量:1
  • 6Bonner-Weir S.Islet growth and development in the adult[J].Mol Endocrinol,2000,24:297. 被引量:1
  • 7Ssaki k.Studies on diabetic cataract in rats induced by streptozotocin[J].Ophthalmic Res,1983,15:185. 被引量:1
  • 8Noguchi H,Kaneto H,Weir GC,et al.PDX-1 protein containing its own antennapedia-like protein transduction domain can transduce pancreatic duct and islet cells[J].Diabetes,2003,52:1732. 被引量:1
  • 9Karlsson O,Edlund T,Moss JB,eta.l Amutational analysis of the insulin gene transcription control region:expression in beta cells is dependent on two related sequenceswithin the enhancer[J].Proc NatlAcad SciUSA,1987,84(24):8819. 被引量:1
  • 10KawamoriD,KajimotoY,KanetoH,eta.l Oxidative stress induces nucleocytoplasmic translocation of pancreatic transcription factor PDX-1 through activation of c-Jun NH(2)-terminal kinase[J].Diabetes,2003,52(12):2896. 被引量:1

同被引文献14

  • 1Vanden -Berghe G, Wilmer A, Hermans G, et al.Intensive insuhn therapy in the medical ICU.N EnglJ Med, 2006, 354(5):449-461. 被引量:1
  • 2Schmidt J, Ratmer DW, Lewandrowski K, et al.A better model of acute pancreatitis for evaluating therapy.Ann Surg, 1992, 215(1):44-56. 被引量:1
  • 3Isaji S, Takada T, Kawarada Y, et al JPN Guidelines for the management of acute pancreatitis:surgical managementJ Hepatobihary Panereat Surg, 2006, 13(1):48-55. 被引量:1
  • 4Van-Cromphaut SJ, Vanhorebeek I, Vanden-Berghe G. Glucose metabolism and insuhn resistance in sepsis. Curt Pharm Des, 2008, 14(19): 1887-1899. 被引量:1
  • 5Handta PD, Mayerb K, Ewald N.Exocrine pancreatic involvement incritically ill patients.Curt Opin Clin Nutr Metab Care, 2009, 12(2): 168-174. 被引量:1
  • 6Barreto SG, Carati CJ, Toouli J, et al.The islet-acinar axis of the pancreas: more than just insulin.Am J Physiol Gastrointest Live physiol, 2010, 299(1):G10-G22. 被引量:1
  • 7Evans JL, Goldfine ID, Maddux BA, et al.Oxidative stress and stress- activated signaling pathways:a uniting hypothesis of type 2 diabetes. Endocr Rev, 2002, 23(4):599. 被引量:1
  • 8Fariss MW, Chan CB, Patel M, et al.P, ole of mitochondria in toxic oxidative stress.Mol Interv, 2005, 5(7):94-99. 被引量:1
  • 9Smelt MJ, Faas MM, De-Haan BJ.Rat pancreatic beta cells and cytomegalovirus infection.Pancreas, 2010, 39(1):47-56. 被引量:1
  • 10Faustino EV, Apkon M.Persistent hyperglycemia in critically ill children.Pediatrics, 2005, 146:30-34. 被引量:1

引证文献1

二级引证文献1

;
使用帮助 返回顶部