摘要
目的通过检测大鼠胰腺组织中Insulin mRNA和PDX-1 mRNA的表达探讨EGF/Gastrin联用促进胰岛PDX-1表达增强可能的作用机制。方法采用一步法实时荧光定量PCR检测各组大鼠胰腺组织中Insulin基因和PDX-1基因在转录水平的表达。结果大鼠胰腺组织中Insulin mRNA在转录水平的表达情况正常对照组最高;糖尿病组最低,与正常对照组相比相差2.4倍(P<0.05);EGF/Gastrin组是糖尿病组的2.1倍(P<0.05)。大鼠胰腺组织中PDX-1mRNA在转录水平的表达情况正常对照组最高,糖尿病组最低,与正常对照组相比相差2.8倍(P<0.05),EGF/Gastrin组是糖尿病组的2.2倍(P<0.05)。结论 EGF/Gastrin联用促进胰岛PDX-1表达增强的作用机制可能通过其改善胰岛β细胞功能、降低血糖进而减弱糖毒性对PDX-1表达的不良影响,间接地使PDX-1的表达增强。而EGF/Gastrin联用促进胰岛新生、改善胰岛β细胞功能又有可能是通过提高PDX-1的表达而实现的。
Objective To discuss the possible mechanism of the PDX-1 expression enhancement caused by combinated use of EGF and Gastrin through detection of insulin mRNA and PDX-1 mRNA genes expression in the pancrease tissue of rats.Methods Evaluate the expression of insulin gene and PDX-1 gene in the transcriptional level with the One-Step Real-Time Fluorescent Quantitative PCR Method.Results The transcription levels of Insulin mRNA in the Control group was the highest while the DM group was the lowest.The transcription level of Insulin mRNA in the Control group was 2.4 times higher than that in the DM group and the level in the EGF/Gastrin group was 2.1 times higher than the DM group.The transcription levels of PDX-1 mRNA in the Control group was the highest while the DM group was the lowest.The transcription level of PDX-1 mRNA in the Control group was 2.8 times higher than that in the DM group and the level in the EGF/Gastrin group was 2.2 times higher than the DM group.Conclusion The combinated use of EGF and Gastrin can promote the expression of PDX-1 by improving pancrease β-cell functions and reducing blood glucose levels to impair the negative effects of glucose toxicity on PDX-1 expression indirectly.However,the mechanism of promotion on the pancrease cells and improvement of pancrease β-cell functions may be realized by the enhancement of PDX-1 expression.
出处
《中国实验诊断学》
北大核心
2011年第8期1269-1273,共5页
Chinese Journal of Laboratory Diagnosis
基金
吉林省卫生厅科研课题(2009Z052)
吉林省科委课题(200505205)