摘要
背景:雌激素受体ERs表达于所有关系到骨形成和骨吸收的细胞成分中。目的:观察雌激素受体ERβ对成骨细胞增殖、分化能力的调控作用。方法:以小鼠成骨细胞株MC3T3-E1为对象,设立3组:实验组转染雌激素受体ERβ RNAi载体、阴性对照组转染ERL RNAi载体、空白对照组不进行转染,3组在相同条件下培养。采用MTT法绘制各组细胞的生长曲线;流式细胞仪分析各组细胞的细胞周期。结果与结论:实验组成骨细胞的增殖能力明显高于空白对照组和阴性对照组,G1期细胞百分率明显少于空白对照组和阴性对照组,而S期和G2期细胞百分率明显高于空白对照组和阴性对照组(P均<0.05)。根据雌激素受体ERβ沉默后检测的结果可以反向推断,ERβ的表达对成骨细胞的增殖、分化具有抑制作用。
BACKGROUND：Estrogen receptors （ERs） are expressed in cell components related to bone formation and resorption. OBJECTIVE：To investigate the regulatory effects of Erβ on osteoblast proliferation and differentiation. METHODS：The mouse osteoblastic cell lines MC3T3-E1 were divided into 3 groups：experimental group （transfected ERβRNAi vector）, negative control （transfected ERL RNAi vector）, and blank control （no transfection）. Three groups of cells were cultured under the same conditions. In each group, cell growth curve was drawn using MTT; and the cell cycle was analyzed by flow cytometry. RESULTS AND CONCLUSION：The cell proliferation in the experimental group was more significantly enhanced than in the negative control group or blank control group. The percentage of cells at G1-phase in the experimental group was significantly less than the other groups, while the percentage of cells at S-phase and G2-phase was significantly higher than the other groups （P 0.05）. According to test results of the ERβ silence, we can infer that the ERβ may inhibit the proliferation and differentiation of osteoblasts.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2011年第28期5157-5160,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
湖南省研究生科研创新项目资助(CX2010B101)~~
