摘要
本研究旨在探讨骨髓增生异常综合征(MDS)患者骨髓异常克隆细胞的起源,即MDS患者骨髓CD34+CD38-造血干细胞和CD34+CD38+祖细胞中是否存在恶性克隆细胞及其比例。用免疫磁珠分选技术(MACS)分选9例染色体异常的MDS患者(8三体4例,含有8三体的复杂核型1例,5q-2例,含有5q-的复杂核型1例,5q-合并8三体1例)的骨髓单个核细胞(BMMNC)中的CD34+CD38-细胞和CD34+CD38+细胞,分别涂片;然后在上述涂片上通过荧光原位杂交(FISH)方法比较CD34+CD38-和CD34+CD38+两群细胞中异常克隆细胞的百分比。结果发现,这两群细胞均有异常克隆累及,但CD34+CD38-干细胞中异常克隆的比例(41.8±8.4)%低于CD34+CD38+祖细胞(72.4±7.7)%(p<0.001),而在有核细胞检测的异常克隆细胞比例为(70.8±9.2)%。结论:提示MDS患者中5q-和+8异常克隆均可能起源于造血干细胞阶段,而在祖细胞阶段异常克隆占优势。
The study was aimed to investigate the origination of abnormal clones in hematopoietic cells of MDS patients. That is to say if there are abnormal clones in CD34 ＋ CD38 - and CD34 ＋ CD38 ＋ cells and their proportions in MDS patients. Immuno-magnetic bead technique was used to sort CD34 ~ CD38 - and CD34 ＋ CD38 ＋ in bone marrow mononuclear cells of 9 MDS patients with chromosome abnormalities （four cases with trisomy 8, 1 case with trisomy 8 complex karyotype, 2 cases with 5q-, 1 case with 5q-complex karyotype, 1 case with 5q- accompanying trisomy 8） and smears were made respectively. Then the percentage of abnormal clones in CD34 ＋ CD38 - and CD34 ＋ CD38 ＋ cells were compared by using FISH. The results indicated that abnormal clones were involved in the two population cells in 9 patients. The percentage of abnormal clones in CD34＋ CD38 - cells （41.8 ± 8.4% ） was obviously lower than that in CD34＋ CD38 ＋ cells （ 72.4 ± 7.7 % ） （p 〈 0.001 ）, and the percentage of abnormal clones in karyocytes was 70.8 ± 9. 2%. It is concluded the abnormal clones of bone marrow hematopoietic cells may originate from stem cell stage in MDS patients with 5q- and ＋ 8, and the abnormal clones are predominant at stage of progenitors.
出处
《中国实验血液学杂志》
CAS
CSCD
2011年第4期930-934,共5页
Journal of Experimental Hematology
