摘要
目的:探讨在大鼠变应性鼻炎(AR)模型单核细胞中T-bet/GATA-3基因的动态表达。方法:20只SD大鼠,雌雄各半,随机分为实验组和对照组,每组各10只。实验组用卵蛋白致敏,以氢氧化铝溶胶和灭活的百日咳杆菌为佐剂,卵蛋白滴鼻建立大鼠AR模型;对照组以氢氧化铝溶胶和灭活的百日咳杆菌生理盐水皮下注射,生理盐水滴鼻。分别在致敏结束、首次激发10h、末次激发10h心脏采血,分离血单核细胞。用RT-PCR法测定单核细胞中T-bet/GATA-3mRNA的表达,对结果进行统计学分析。结果:致敏结束、首次激发10h、末次激发10h3个时间点实验组T-bet mRNA的相对表达量分别为0.404±0.187、1.676±0.708、0.503±0.514,GATA-3mRNA的相对表达量分别为0.434±0.147、0.600±0.480、1.029±0.690;对照组T-bet mRNA的相对表达量分别为0.487±0.212、0.486±0.148、0.495±0.103,GATA-3mRNA的相对表达量分别为0.596±0.249、0.474±0.101、0.550±0.119。实验组首次激发后10hT-bet mRNA相对表达量明显高于对照组、末次激发后10h和致敏结束(t=4.18、5.14、5.27,均P<0.01),实验组末次激发后10hGATA-3mRNA的相对表达量明显高于致敏结束时、首次激发后10h及对照组(t=3.51、2.53、2.71,均P<0.05),对照组在上述3个时间点GATA-3mRNA和T-bet mRNA的相对表达量均无明显变化。结论:AR的发生、发展是一个程序化、动态的过程,GATA-3和T-bet表达比例失衡可能是AR发生的基因基础,二者在AR发生、发展中均起一定作用,只是表达的时限不同。
Objective：To investigate kinetic expression of genes T-bet and GATA-3 in blood mononuclear cells（BMCs）in allergic rhinitis rats model at different stages of development of allergic rhinitis.Method：Twenty SD rats（including 10 male and 10 female）were divided into 2 groups,experimental group and control group,randomly,10 rats for each group.Ten rats in experimental group were sensitized and intranasally challenged by OVA,aluminium hydroxide hydrate gel and Bordelella pertussis inactive microorganism suspension（B.pertussis） adjuvants,as allergic rhinitis models,Ten rats in control group were investigated using physiological saline only.BMCs were separated from 2 ml blood which was extracted from rat heart at the end of sensitization,10-hour after the first challenge and 10-hour after the final challenge,respectively.RT-PCR was utilized to detect the expression of T-bet and GATA-3.Result：At the end of sensitization,10-hour after the first challenge and 10-hour after the final challenge,in experimental group,Relative quantitation of expression of T-bet was 0.404±0.187,1.676±0.708,0.503±0.514 and that of GATA-3 was 0.434±0.147,0.600±0.480,1.029±0.690,respectively.While,In control group,Relative quantitation of expression of T-bet was 0.487±0.212,0.486±0.148,0.495±0.103 and and its of GATA-3 was 0.596±0.249,0.474±0.101,0.550±0.119,respectively.At 10-hour after the first challenge,relative quantitation of expression of T-bet in experimental group was increased markedly and there was significant difference compared with contol group（t=4.18,P0.01）.In experimental group,The amount of expression of T-bet at 10-hour after first challenge was higher than it at 10-hour after the final challenge and there was markedly difference（t=5.14,P0.01）.The amount of expression of T-bet at 10-hour after first challenge was increased significantly compared with it at the end of sensitization（t=5.27,P0.01）.while,the expression of GATA-3 at 10-hour after final challenge was markedly increased compared
出处
《临床耳鼻咽喉头颈外科杂志》
CAS
CSCD
北大核心
2011年第17期791-795,共5页
Journal of Clinical Otorhinolaryngology Head And Neck Surgery
基金
广东省自然科学基金(No:8151008901000105)
