摘要
目的:观察RYBP(Ring1 and YY1-binding protein)在急性白血病中的表达情况,初步探索其在急性白血病中的临床意义。方法:应用蛋白质免疫印迹(western blot)技术分别检测急性单核细胞白血病细胞株SHI-1、急性早幼粒白血病细胞株HL-60、慢性粒细胞白血病细胞株K562、51例初治急性白血病(AL)患者、23例急性白血病完全缓解(ALCR)患者和21例对照(非恶性血液病)患者骨髓单个核细胞中RYBP的表达情况。结果:SHI-1、HL-60及K562细胞株RYBP表达均为强阳性;21例阴性对照中RYBP表达均为阴性。AL组RYBP表达阳性率为73%,ALCR组阳性率为13%,AL组RYBP表达阳性率及表达量比ALCR组及对照组明显增高(P<0.05)。结论:RYBP在急性白血病细胞中表达水平异常增高,提示RYBP可能与急性白血病密切相关。
Objective To investigate the expression and its significance of RYBP （Ringl and YYl-binding protein）in acute leukemia （AL）. Methods RYBP level was assessed by Western blot in the bone marrow mononuclear cells （BM-MNCs） of 51 acute leukemia patients （AL）, 23 complete remission （ALCR） patients and 21 negative controls, as well as leukemia cell line SHI-1, HL-60 and K562. Results The expression of RYBP was positive in leukemia cell line SHI-1, HL-60 and K562 but negative in 21 negative controls. The RYBP was expressed in 73% AL patients and 13% ALCR patients. The expression rate and level of RYBP in AL patients was significantly higher than that in ALCR patients （P 〈 0.05）. Conclusion RYBP was over-expressed in acute leukemia patients, suggesting that RYBP is associated with acute leukemia.
出处
《实用医学杂志》
CAS
北大核心
2011年第18期3301-3303,共3页
The Journal of Practical Medicine
基金
国家自然科学基金资助项目(编号:30871101)
广州市医药卫生科技重点资助项目(编号:2006-ZDi-02)
2009年度广州市留学人员专项资金资助项目
