摘要
目的:建立人偏肺病毒(Human metapneumovirus,hMPV)感染的哮喘小鼠模型,验证玉屏风散对hMPV诱导的哮喘小鼠气道高反应性和炎症的疗效并初步探讨其机理。方法:随机将48只雌性BALB/c小鼠分为对照组(A组)、OVA致敏激发哮喘组(B组)、偏肺病毒哮喘组(C组)、小剂量玉屏风组(D组)、中剂量玉屏风组(E组)、大剂量玉屏风组(F组),采用卵蛋白致敏和激发,hMPV滴鼻方法建立哮喘人偏肺病毒感染模型并给予玉屏风治疗,采用动物体描箱法测定气道反应性;采用支气管肺泡灌洗液(BALF)进行细胞分类计数;肺组织病理切片HE染色观察炎性细胞浸润;采用流式细胞术分别测定肺部细胞因子IFNγ-和IL-4、IL-17。结果:(1)随吸入乙酰甲胆碱浓度增加各组气道反应性明显增加,C组比B组气道反应性显著升高(P<0.05);F组气道反应性明显降低,低于C组(P<0.05);(2)各组BALF中白细胞总数及炎症细胞数都比对照组显著升高,C组BALF中白细胞总数、嗜酸性粒细胞与B组、F组相比显著升高(P<0.05);(3)各哮喘组的炎症评分都显著高于对照组。在细支气管周围炎、肺泡炎方面B组和F组比C组明显减轻(P<0.05)。(4)A、B组IFNγ-无差异(P>0.05),各剂量组IFNγ-,IFNγ-/IL-4均显著高于B、C组(P<0.05);哮喘组、治疗组IL-4显著高于正常组(P<0.01),但各组间无明显差异;与B组相比,C组IL-17水平明显升高(P<0.05);与C组相比,E、F组IL-17水平明显降低。结论:大剂量玉屏风散可以抑制感染hMPV哮喘小鼠的气道高反应性及气道炎症,且改善Th1/Th2失衡状态。
Objective：To investigate the impact of Yu-Ping-Feng power on the airway responsiveness and inflammatory in an asthmatic mouse model infected by human metapneumovirus and the possible underlying mechanisms.Methods：Forty-eight BALB/c mice were randomly divided into 6 groups.All the mice except control group and OVA group were inoculated intranasally with hMPV after underwent ovalbumin（OVA） sensitization and challenge,then being orally lavaged with various doses of Yu-Ping-Feng for 1 week.Airway responsiveness to inhaled methamholine was measured and bronchoalveolar lavage（BAL） was performed after the last challenge.Cells in BAL fluid（BALF） was counted and inflammatory characteristics of lungs was scored by staining with hematoxylin and eosin.Cytokines of lungs were detected by flow cytometry.Results：（1） OVA＋hMPV group and low dose group displayed the highest airway responsiveness,significant difference was found between OVA group and OVA＋hMPV group（P0.05）.High dose group significantly reduced airway responsiveness,lower than OVA＋hMPV group.（2） The numbers of total white cells and eosinophils in the BALF from OVA＋hMPV group was significantly greater than control group,OVA group and high dose group（P0.05）;（3）Histological score of peribronchiolitis,alveolitis and perivasculitis in all OVA-sensitized/challenged groups were significantly higher than that in control.OVA group and high dose group had significantly milder peribronchiolitis and alveolitis than OVA＋hMPV group.（4）There was no difference in IFN-γ level between control group and OVA group.IFN-γ,IFN-γ/IL-4 of each treatment group was significantly higher than that of OVA＋hMPV group.IL-4 of All OVA-sensitized/challenged groups significantly higher than that of the control group（P0.01）,but no significantly difference among the groups.IL-17 of OVA＋hMPV group was significantly higher than that of OVA group,media dose group and high dose group.Conclusion：Yu-Ping-Feng can inhibit the airway hyperresponsi
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2011年第8期709-713,共5页
Chinese Journal of Immunology
基金
国家自然科学基金重点项目基金(30730098)
重庆市杰出青年科学基金(CSCT
2008BA5040)
关键词
玉屏风散
人偏肺病毒
哮喘
气道反应性
Yu-Ping-Feng powers
Human metapneumovirus
Asthma
Airway responsiveness
