摘要
目的:了解鼠疫菌在历史鼠疫患者坟墓土壤中的存在状况。方法:采用赫氏培养基对鼠疫菌进行培养,同时利用Real-time定量荧光PCR对样本进行定性分析。结果:病原培养及荧光定量PCR定性分析显示样本均为阴性。结论:鼠疫菌在历史鼠疫患者坟墓的土壤中无法存活64年。
Objective：To deeply understand existing state of the Yersinia pestis in grave soil of a deceased plague patient.Methods：Yersinia pestis were cultured on the hershey medium;qualitative analysis of samples were performed by Real-time fluorescence PCR.Results：Pathogen culture and real-time quantitative PCR analysis showed that samples were negative.Conclusion：Yersinia pestis could not survive for 64 years in grave soil of the dead plague patients.
出处
《中国卫生检验杂志》
CAS
2011年第8期1954-1955,共2页
Chinese Journal of Health Laboratory Technology
关键词
坟墓
土壤
鼠疫菌
检测
Grave
Soil
Yersinia pestis
Detection
