摘要
为了挖掘水稻中的逆境诱导型启动子,挑选了1个受干旱胁迫强烈诱导的水稻内源基因Oshox24,分离出该基因的启动子(命名为Oshox24P),通过酶切连接的方法构建GUS报告基因表达载体并转化到受体材料中花11中,通过对转基因后代在不同逆境胁迫下的GUS活性检测,证明该启动子强烈地受到干旱胁迫的诱导,上调表达倍数至十几倍,同时该启动子还受到脱落酸(ABA)的诱导,对高盐和低温胁迫的反应并不明显。结果表明,该启动子属于干旱诱导型启动子,可以用于控制目标基因在水稻中的表达。
Abiotic stresses are major limiting factors for crops growth and production,and stress resistance of crops can be significantly improved by overexpressing stress-related genes.In this study we aimed to isolate and identify a stress-inducible promoter which is useful for genetic engineering.Firstly a candidate promoter Oshox24P was screened and fused to GUS expression vector,and then transferred into japonica rice Zhonghua 11.GUS activity in transgenic rice plants containing GUS reporter gene under the control of Oshox24P promoter（1 918 bp in length） was induced by drought and the activity increased in response to the increasing severity of drought stress.The GUS activity was slightly induced by abscisc acid（ABA） while treatments of salt and cold had little effect on Oshox24 promoter-driven GUS expression.These results suggest that Oshox24P is a drought-inducible promoter,and will be useful for driving specific expression of target genes or proteins during drought stress,thus for genetic improvement of drought resistance.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2011年第5期525-531,共7页
Journal of Huazhong Agricultural University
基金
国家转基因重大专项(2008ZX08009-004)
