摘要
以大豆子叶节作为外植体,通过农杆菌介导法将含有BrCS基因的pCAMBIA3300的双子叶植物表达载体,导入到大豆品种黑农59和黑农53中,并对黑农59遗传转化的影响因素进行了优化,最终获得了抗性植株。经PCR检测和PCR-Southern杂交分析,初步证明了目的基因已整合到大豆的基因组中。
In this study,plant expression vector pCAMBIA3300-BrCS containing-BrCS-gene was constructed,and transgenic plants were obtained by Agrobacterium-mediated transformation of soybean（Heinong 59 and Heinong53）cotyledonary nodes.We regenerated plants and optimized the conditions of transformation to increase the efficiency of transformation.PCR and PCR-Southern detection was used to identify the integrated level of BrCS gene in transgenic plants;it confirmed the integration of BrCS genes into the genome of soybean.
出处
《大豆科学》
CAS
CSCD
北大核心
2011年第4期569-573,共5页
Soybean Science
基金
国家转基因重大专项资助项目(2008ZX08004-002)
现代农业产业技术体系资助项目(nycytx-004)
关键词
大豆
遗传转化
BrCS基因
抗冷性
耐盐性
Soybean
Genetic transformation
BrCS gene
Cold-stress tolerance
Salt-stress tolerance
