摘要
目的探讨粤西地区汉族人群中生长停滞特异性基因6(GAS6基因)内含子SNP344-35C/T位点的单核苷酸多态性(SNP)与脑梗死的相关性。方法应用聚合酶链反应-限制性内切酶片段长度多态性分析方法(PCR-RFLP)检测180例脑梗死患者和150例健康对照的GAS6基因内含子SNP344-35C/T的基因型。结果 GAS6基因内含子SNP344-35C/T基因型及等位基因频率在病例组与对照组之间无显著性差异(P>0.05),经性别分层分析发现,女性C等位基因频率(77.9%)高于对照组(66.4%)(P=0.040);CC基因型频率(61.8%)高于对照组(43.1%)(P=0.036)。结论 GAS6基因内含子SNP344-35C/T位点与粤西地区汉族女性脑梗死有关,C等位基因是女性脑梗死的危险因素,CC基因型为女性脑梗死的易感基因型。
Objective To investigate the correlations of the single nucleotide polymorphism （SNP） 344-35C/T of growth arrest-specific gene 6 （GAS6） gene and cerebral infarction in Han population in the western Guangdong province. Methods The restriction fragment length polymorphism （PCR-RFLP） was used to determine the 344-35C/T polymorphism in the intron region of GAS6 gene in the case group （n= 180） and healthy control group （n= 150）. Results There was no significant association between GAS6-344-35C/T and cerebral infarction （P〉 0.05）. After stratified by gender in chi-square test in women population, the frequency of C allele was significantly higher in cases （77.9%） than that in controls （66.4%） （P=0.040）; The frequency of the CC genotype was significantly higher in cases （61.8%） than that in controls （43.1%） （P=0.036）. Conclusion In Han women in western Guangdong province, C allele of GAS6-344-35C/T polymorphism is a risk factor of cerebral infarction, CC genotype is a susceptible genotype of cerebral infarction.
出处
《中国康复理论与实践》
CSCD
2011年第8期701-704,共4页
Chinese Journal of Rehabilitation Theory and Practice
基金
广东湛江市科技项目:卒中单元的建设与推广
关键词
脑梗死
生长停滞特异性基因6
单核苷酸多态性
cerebral infarction
growth arrest-specific gene 6
single nucleotide polymorphism
