摘要
采用PCR方法从微小隐孢子虫基因组中扩增Cp735基因。与pMD-18T载体连接后,挑取阳性重组子测序分析。用基因重组技术将Cp735基因克隆入原核表达载体pET-28a中,构建Cp735基因的重组原核表达载体pET28a-Cp735。将pET28a-Cp735在大肠杆菌BL21(DE3)中诱导表达,并用SDS-PAGE和Western blot进行鉴定。结果表明:得到了在大肠杆菌中高效表达的重组蛋白,分子质量大小约为26 kD,并且具有反应原性。
The Cp735 gene was amplified by PCR.Coding sequence identified by DNA sequencing was cloned into the expression vector pET-28a to construct prokaryotic expression vector.The recombinant protein was expressed in BL21（DE3）.The result of SDS-PAGE showed that the molecular weight of the expressed product was identical to that of prediction.The result of Western blot demonstrated that the expressed protein had reactogenicity.
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2011年第5期567-570,共4页
Journal of Jilin Agricultural University
基金
国家科技支撑计划项目(2007BAD40B05)
国家传染病防治重大专项(2008ZX10004-001-B)
