摘要
利用响应面分析法对超声波提取沙棘叶水溶性多糖的工艺进行优化。在单因素试验基础上,选择超声温度、液料比、超声功率和超声时间为自变量,以多糖提取率为响应值,采用中心组合设计的方法,研究各自变量及其交互作用对多糖提取率的影响。利用Design Exepert 8.0.32分析确定最佳提取工艺,得出超声波提取沙棘叶水溶性多糖工艺的最佳条件为超声水浴温度80℃、液料比34∶1、超声功率300 W、超声时间32 min。在此条件下,多糖提取率为9.94%,验证试验值为9.91%。
To optimize ultrasonic wave extraction of water soluble polysaccharides from foliage in Hippophae Rhamnoides L.（WPFH）,single factor experiments involving ultrasonic temperature,water-feed ratio,ultrasonic power,ultrasonic time were studied for following investigation on the interactive effects of the parameters on WPFH yield using Box-Benhnken central composite design（BBD） combined with response surface methodology（RSM）.Design Expert 8.0.3.2 software was used to fit BBD experimental data and a quadratic polynomial regression model was developed for WPFH yield as a response to the above parameters.The results showed that the optimum conditions for WPFH extraction were as follows： ultrasonic temperature 80℃,water-feed ratio 34∶1,ultrasonic power 300 W and ultrasonic time 32 minutes.And the extraction yield of WPFH was 9.94% under this condition.The actual value was 9.91%.
出处
《广东农业科学》
CAS
CSCD
北大核心
2011年第12期101-104,共4页
Guangdong Agricultural Sciences
基金
辽宁工程技术大学理学院优秀青年科学研究基金(07-127)
关键词
沙棘叶
多糖
超声提取
响应面分析
Sea Buckthorn leaves
polysaccharide
ultrasonic wave extraction
response surface analysis
